麦门冬汤加减方联合顺铂对Lewis肺癌移植瘤模型小鼠的抑瘤作用及机制研究
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篇名: 麦门冬汤加减方联合顺铂对Lewis肺癌移植瘤模型小鼠的抑瘤作用及机制研究
TITLE:
摘要: 目的:研究麦门冬汤加减方联合顺铂对Lewis肺癌移植瘤模型小鼠的抑瘤作用,并探究其可能的作用机制。方法:通过腋下接种Lewis肺癌细胞复制Lewis肺癌移植瘤小鼠模型。将60只小鼠随机分为模型组(生理盐水,每天灌胃1次)、顺铂组(6 mg/kg,每周腹腔注射1次)、麦门冬汤加减方组(20 g/kg,每天灌胃1次)和联合组(每周腹腔注射6 mg/kg顺铂1次+每天灌胃20    g/kg麦门冬汤加减方1次),每组15只。所有小鼠均连续给药2周。给药结束后,检测各组小鼠瘤质量和胸腺指数,苏木精-伊红(HE)染色后观察其瘤体组织病理变化,TUNEL法检测其瘤体细胞凋亡指数,Western blot法检测其瘤体组织中B细胞淋巴瘤2(Bcl-2)和促凋亡基因Bax蛋白表达。结果:与模型组比较,麦门冬汤加减方组、顺铂组和联合组小鼠瘤质量、Bcl-2蛋白表达量均显著降低(P<0.05),胸腺指数、瘤体细胞凋亡指数、Bax蛋白表达量均显著升高(P<0.05);并且联合组小鼠瘤质量、Bcl-2蛋白表达量明显低于麦门冬汤加减方组和顺铂组(P<0.05),胸腺指数、瘤体细胞凋亡指数、Bax蛋白表达量明显高于麦门冬汤加减方组和顺铂组(P<0.05)。HE染色结果显示,麦门冬汤加减方组、顺铂组和联合组小鼠肿瘤细胞密度有所降低,肿瘤组织坏死区域增多,并且联合组小鼠的坏死区面积明显大于麦门冬汤加减方组和顺铂组。结论:麦门冬汤加减方可通过下调瘤组织中Bcl-2蛋白表达和上调Bax蛋白表达,从而抑制Lewis肺癌移植瘤模型小鼠肿瘤的生长。
ABSTRACT: OBJECTIVE: To study the inhibitory effect of modified Maimendong decoction combined with cisplatin on Lewis lung cancer transplantation model mice, and to explore its potential mechanism. METHODS: Lewis lung cancer transplantation mice model was induced via subaxillary inoculation of Lewis lung cancer cells. 60 mice were randomly divided into model group (normal saline, once a day, i.g.), cisplatin group (6 mg/kg, once a week, i.p.), modified Maimendong decoction group (20 g/kg. once a day, i.g.) and combination group (cisplatin 6 mg/kg, once a week, i.p.+modified Maimengdong decoction, once a day, i.g.), with 15 mice in each group. All mice were treated for consecutive 2 weeks. After medication, tumor weight and thymus index were detected; HE staining was used to observe the pathological change of tumor tissue. TUNEL was used to detect apoptotic index of tumor tissue. The protein expressions of Bcl-2 and Bax were detected by Western blot assay. RESULTS: Compared with model group, tumor weight and protein expression of Bcl-2 were decreased significantly in modified Maimengdong decoction, cisplatin group and combination group (P<0.05), and thymus index, tumor apoptotic index and the protein expression of Bax were increased significantly (P<0.05). Tumor weight and protein expression of Bcl-2 in combination group were significantly lower than modified Maimengdong decoction group and cisplatin group (P<0.05); thymus index, tumor apoptotic index and the protein expression of Bax were significantly higher than addition and subtraction of modified Maimengdong decoction group and cisplatin group (P<0.05). HE staining showed that the density of tumor cells was decreased to certain degree in modified Maimengdong decoction group, cisplatin group and combination group; the area of necrosis area in the combination group was significantly larger than in modified Maimendong decoction group and cisplatin group. CONCLUSIONS: Modified Maimendong decoction can inhibit the growth of Lewis lung cancer transplanted tumor in mice by down-regulating the protein expression of Bcl-2 and up-regulating the protein expression of Bax.
期刊: 2019年第30卷第9期
作者: 方芳,吴万垠,杨春旭,宁雪坚,谢伶俐,斯韬
AUTHORS: FANG Fang,WU Wanyin,YANG Chunxu,NING Xuejian,XIE Lingli,SI Tao
关键字: 麦门冬汤加减方;顺铂;Lewis肺癌;移植瘤;B细胞淋巴瘤2;小鼠
KEYWORDS: Modified Maimendong decoction; Cisplatin; Lewis lung cancer; Transplanted tumor; Bcl-2; Mice
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