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不同配比人参总皂苷、丹皮总苷、丹皮酚含药血清对H2O2诱导的人脐静脉内皮细胞损伤的改善作用研究
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| 篇名： | 不同配比人参总皂苷、丹皮总苷、丹皮酚含药血清对H2O2诱导的人脐静脉内皮细胞损伤的改善作用研究 |
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| 摘要： | 目的：研究不同配比的人参总皂苷（TGG）、丹皮总苷（TGM）、丹皮酚含药血清对H2O2诱导的人脐静脉内皮细胞（HUVEC）损伤的改善作用，筛选最优配比并探讨其作用机制。方法：将大鼠随机分为空白组（蒸馏水）、TGG组（TGG，2.025 g/kg）、TGM组（TGM，4.05 g/kg）、丹皮酚组（丹皮酚，1.08 g/kg），每组12只，每天灌胃相应药物2次，连续7 d，末次给药后1 h，腹主动脉取血，制备含药血清。以HUVEC细胞存活率为评价指标，人参总皂苷、丹皮总苷、丹皮酚含药血清不同配比为考察因素设计L9（34）正交试验，优选3种含药血清的最优配比；将HUVEC细胞分为空白组、模型组、TGG组、TGM组、丹皮酚组、最优配比组，除空白组细胞加入相应培养基外，其余各组均采用1.2 mmol/L H2O2诱导HUVEC损伤，然后TGG组（含药血清体积分数为0.000 5％）、TGM组（含药血清体积分数为0.000 5％）、丹皮酚组（含药血清体积分数为1％）、最优配比组再加入相应药物含药血清进行干预。采用微板法和酶联免疫吸附试验（ELISA）法检测各组细胞中乳酸脱氢酶（LDH）、一氧化氮（NO）、内皮素1（ET-1）的水平。结果：含药血清最优配比为TGG 0.000 5％、TGM 0.000 5％、丹皮酚1％。与空白组比较，模型组细胞LDH、ET-1水平更高（P＜0.01），NO水平更低（P＜0.05）；与模型组比较，TGG、TGM、最优配比组细胞NO水平更高（P＜0.01），LDH、ET-1水平更低（P＜0.05或P＜0.01）；与TGG组、TGM组、丹皮酚组比较，最优配比组细胞LDH水平更低（P＜0.05或P＜0.01），NO水平更高（P＜0.05或P＜0.01）。结论：TGG、TGM、丹皮酚联合应用对H2O2诱导的HUVEC损伤具有良好的改善作用，其作用机制与降低LDH、ET-1水平和升高NO水平有关。 |
| ABSTRACT： | OBJECTIVE： To study improvement effects of different proportions of total glucosides of ginseng （TGG）， total glucosides of moutan cortex （TGM） and paeonol containing serum on the injury of human umbilical vein endothelial cells （HUVEC） injury induced by hydrogen peroxide （H2O2）， screen the optimal proportion and investigate its mechanism. METHODS： The rats were randomly divided into blank group （distilled water）， TGG group （TGG， 2.025 g/kg）， TGM group （TGM， 4.05 g/kg） and paeonol group （paeonol， 1.08 g/kg）， with 12 rats in each group. They were given relevant medicine twice a day for consecutive 7 days. 1 h after last medication， the blood samples were collected via abdominal aorta to prepare drug containing serum. Using survival rate of HUVEC as evaluation indexes， different proportions of TGG， TGM and paeonol containing serum as factors， L9（34） orthogonal test was designed to optimize the optimal proportion of 3 kinds of drug containing serum. HUVEC were divided into blank group， model group， TGG group， TGM group， paeonol group and optimal proportion group. Except that blank group were treated with relevant medium， other group were treated with 1.2 mmol/L H2O2 to induce HUVEC injury， and then TGG group （volume fraction of drug containing serum was 0.000 5％）， TGM group （volume fraction of drug containing serum was 0.000 5％）， paeonol group （volume fraction of drug containing serum was 1％） and optimal proportion group were intervened with drug containing serum. The levels of LDH， NO and ET-1 in cells were detected by microplate method and ELISA. RESULTS： The optimal proportion of drug containing serum were TGG 0.000 5％， TGM 0.000 5％ and paeonol 1％. Compared with blank group， the levels of LDH and ET-1 were higher in model group （P＜0.01）， while NO level was lower （P＜0.05）. Compared with model group， the levels of NO were higher in TGG group， TGM group and optimal proportion group （P＜0.01）， while the levels of LDH and ET-1 were lower （P＜0.05 or P＜0.01）. Compared with TGG group， TGM group and paeonol group， the level of LDH was lower in optimal proportion group （P＜0.05 or P＜0.01）， while the level of NO was higher （P＜0.05 or P＜0.01）. CONCLUSIONS： TGG and TGM combined with paeonol can significantly improve HUVEC injury induced by H2O2， and the mechanism of which may be associated with the decrease of LDH and ET-1 and the increase of NO. |
| 期刊： | 2019年第30卷第9期 |
| 作者： | 张留记，王建霞，李开言，屠万倩 |
| AUTHORS： | ZHANG Liuji，WANG Jianxia，LI Kaiyan，TU Wanqian |
| 关键字： | 人参总皂苷；丹皮总苷；丹皮酚；联合应用；含药血清；配比；人脐静脉内皮细胞 |
| KEYWORDS： | Total glucosides of ginseng； Total glucosides of moutan cortex； Paeonol； Combined use； Drug containing serum； Proportion； Human umbilical vein endothelial cell |
| 阅读数： | 296 次 |
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