蛇葡萄素与8种抗生素联用的体外抗铜绿假单胞菌作用研究
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篇名: 蛇葡萄素与8种抗生素联用的体外抗铜绿假单胞菌作用研究
TITLE:
摘要: 目的:研究蛇葡萄素与8种抗生素联用的体外抗铜绿假单胞菌作用。方法:采用棋盘式微量稀释法测定蛇葡萄素分别与头孢曲松、头孢哌酮/舒巴坦、哌拉西林/他唑巴坦、头孢哌酮、环丙沙星、阿米卡星、哌拉西林、头孢吡肟8种抗生素联用对铜绿假单胞菌(PA)标准菌株ATCC27853和7株临床分离菌株PA135、PA216、PA276、PA281、PA291、PA314、PA319的最小抑菌浓度(MIC),以分级抑菌浓度指数(FICI)评价其联用效果;并以临床分离菌株PA319为目的菌株,设置正常对照组、蛇葡萄素单用组、抗生素单用组以及二者联用组,以联用时蛇葡萄素与抗生素的MIC为作用浓度,计数作用0、4、8、12、24 h的菌落数,绘制时间-杀菌曲线。结果:针对上述8株菌株,蛇葡萄素单用的MIC为128~256 mg/L,分别与头孢曲松、头孢哌酮/舒巴坦、哌拉西林/他唑巴坦、头孢哌酮、环丙沙星、阿米卡星、哌拉西林、头孢吡肟联用时FICI≤1的分别有8、8、7、6、4、4、6、6株。在时间-杀菌曲线中,与抗生素单用比较,蛇葡萄素分别与头孢曲松、头孢哌酮/舒巴坦、哌拉西林/他唑巴坦、头孢哌酮、环丙沙星、阿米卡星、哌拉西林、头孢吡肟联用后菌落数的下降幅度分别为2.65、2.30、0.42、0.47、0.53、1.19、1.74、1.04    lgCFU/mL。结论:蛇葡萄素与头孢曲松、头孢哌酮/舒巴坦联用时对铜绿假单胞菌的抗菌效果更好。
ABSTRACT: OBJECTIVE: To study the antimicrobial activity of ampelopsin combined with 8 kinds of antibiotic against Pseudomonas aeruginosa (PA) in vitro. METHODS: Chessboard trace dilution method was used to determine minimum inhibitory concentration (MIC) of ampelopsin combined with ceftriaxone, cefoperazone/sulbactam, piperacillin/tazobactam, cefoperazone, ciprofloxacin, amikacin, piperacillin and cefepime to PA strain ATCC27853 and 7 isolated strains PA135, PA216, PA276, PA281, PA291, PA314 and PA319. Fractional inhibitory concentration index (FICI) was used to evaluate its effects of drug combination. Clinically isolated strain PA319 were taken as target strain and then divided into normal control group, ampelopsin alone group, antibiotics alone group and ampelopsin+antibiotic combination group. Using MIC of ampelopsin and antibiotics during drug combination as active concentration, the number of colonies cultured for 0, 4, 8, 12 and 24 h was counted, and the time-sterilization curve was drawn. RESULTS: For above 8 kinds of strains, MIC of ampelopsin alone was 128-256 mg/L; FICI of ampelopsin combined with ceftriaxone, cefoperazone/sulbactam, piperacillin/tazobactam, cefoperazone, ciprofloxacin, amikacin, piperacillin and cefepime to 8, 8, 7, 6, 4, 4, 6 and 6 strains were equal to or lower than 1, respectively. In time-antibacterial curve, compared with antibiotics alone, the number of colonies decreased by 2.65, 2.30, 0.42, 0.47, 0.53, 1.19, 1.74, 1.04 lgCFU/mL respectively after ampelopsin combined with ceftriaxone, cefperazone/sulbactam, piperacillin/tazobactam, cefoperazone, ciprofloxacin, amikacin, piperacillin and cefepime. CONCLUSIONS: Ampelopsin combined with ceftriaxone and cefoperazone/sulbactam show better antibacterial effect on PA.
期刊: 2019年第30卷第1期
作者: 谢俊杰,阮兆娟,左国营
AUTHORS: XIE Junjie,RUAN Zhaojuan,ZUO Guoying
关键字: 蛇葡萄素;铜绿假单胞菌;临床分离菌株;抗菌作用
KEYWORDS: Ampelopsin; Pseudomonas aeruginosa; Clinically isolated strain; Antibacterial effect
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