18F-FDG PET/CT技术监测雷公藤甲素对类风湿关节炎模型大鼠关节损伤的改善作用
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篇名: 18F-FDG PET/CT技术监测雷公藤甲素对类风湿关节炎模型大鼠关节损伤的改善作用
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摘要: 目的:建立监测雷公藤甲素对类风湿关节炎模型大鼠关节损伤改善作用的方法。方法:将18只SD大鼠随机分为正常对照组(0.9%氯化钠溶液)、模型组(0.9%氯化钠溶液)、雷公藤甲素组(0.5 mg/kg),每组6只。模型组和雷公藤甲素组大鼠尾根部皮下注射不完全弗氏佐剂-鸡Ⅱ型胶原蛋白(1 ∶ 1,V/V)0.1 mL进行初次免疫,7 d后进行第二次免疫,以建立类风湿关节炎模型。造模成功后各给药组大鼠均尾静脉注射相应药物,正常对照组和模型组大鼠均给予等体积0.9%氯化钠溶液,每2 d 1次,连续30 d。分别于给药前(0 d)和给药5、10、15、20、25、30 d后称定大鼠体质量;于给药前(0 d)和给药3、6、9、12、15、18、21、24、27、30 d后对大鼠进行关节炎指数评分;于给药6、18、30 d后利用正电子发射计算机断层显像(PET)/计算机断层扫描(CT)系统扫描大鼠骨关节,扫描50 min前大鼠尾静脉注射500 μCi的18F-氟代脱氧葡萄糖(18F-FDG),PET显像采用滤波反投影图像重构算法;采用CT扫描进行标准定位;采用Inveon Research Workplace 4.2分析软件计算病变部位的最大标准摄取值(SUV)。结果:与正常对照组比较,给药15~30 d后,模型组大鼠体质量均显著减轻;给药6~30 d后,模型组大鼠关节炎指数评分均显著升高;给药6、18、30 d后,模型组大鼠病变部位SUV均显著升高(P<0.05);模型组大鼠关节炎病变逐渐加重,并出现骨质破坏情况。与模型组比较,给药15~30 d后,雷公藤甲素组大鼠体质量均显著增加;给药21~30 d后,雷公藤甲素组大鼠关节炎指数评分显著降低;给药18、30 d后,雷公藤甲素组大鼠病变部位SUV均显著降低(P<0.05);雷公藤甲素组大鼠关节炎病变逐渐减轻。结论:18F-FDG PET/CT技术能有效监测雷公藤甲素对类风湿关节炎模型大鼠关节损伤的改善作用。
ABSTRACT: OBJECTIVE: To establish the method for detect the improvement effects of triptolide (TP) on joint injury in rheumatoid arthritis (RA) model rats. METHODS: Totally 18 SD rats were randomly divided into normal control group (0.9% Sodium chloride solution), model group (0.9% Sodium chloride solution) and TP group (0.5 mg/kg), with 6 rats in each group. Model group and TP group were given incomplete Freund adjuvant-chicken type II collagen (1 ∶ 1,V/V) 0.1 mL subcutaneously via tail for initial immunity, and 7 d later given secondary immunity to established RA model. After modeling, they were given relevant medicine intravenously via tail vein; normal control group and model group were given constant volume of 0.9% Sodium chloride solution, every 2 d, for consecutive 30 d. Body weights of rats were weighed before medication, 5, 10, 15, 20, 25, 30 d after medication. Arthritis indexes of rats were scored before medication, 3, 6, 9, 12, 15, 18, 21, 24, 27, 30 d after medication. Positron emission computed tomography (PET)/tomography (CT) were used to scan the joint of rats 6, 18, and 30 d after administration. 18F-FDG 500 μCi was injected into the tail vein 50 min before scanning. The filtered back projection image reconstruction algorithm was adopted for PET imaging. CT scanning was used for standard positioning. Inveon Research Workplace 4.2 analysis software was used to calculate the maximum standard uptake value (SUV) of lesions. RESULTS: Compared with normal control group, 15-30 d after medication, body weight of rats was decreased significantly in model group; 6-30 d after medication, arthritis index scores of rats were increased significantly; 6, 18, 30 d after medication, SUV of lesions in rats increased significantly (P<0.05), and the severity of arthritis in rats was aggravated and bone destruction occurred. Compared with model group, 15-30 d after medication, body weight of rats increased significantly in TP group; 21-30 d after medication, arthritis index score of rats decreased significantly; 18, 30 d after medication, SUV of lesion decreased significantly (P<0.05), and arthritis relieved gradually. CONCLUSIONS: 18F-FDG PET/CT imaging is an efficient method to monitor the improvement effect of TP on joint injury in RA model rats.
期刊: 2018年第29卷第22期
作者: 冯悦,钟萌,罗见春,万胜利,叶云,陈跃
AUTHORS: FENG Yue,ZHONG Meng,LUO Jianchun,WAN Shengli,YE Yun,CHEN Yue
关键字: 雷公藤甲素;类风湿关节炎;大鼠;正电子发射计算机断层显像;计算机断层扫描;18F-氟代脱氧葡萄糖;最大标准摄取值
KEYWORDS: Triptolide; Rheumatoid arthritis; Rats; Positron emission computed tomography; Tomography; 18F-FDG; Maximum standard uptake value
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