蛇葡萄素对皮离蛋白过表达的大肠癌LoVo细胞迁移和侵袭能力的抑制作用研究
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篇名: 蛇葡萄素对皮离蛋白过表达的大肠癌LoVo细胞迁移和侵袭能力的抑制作用研究
TITLE:
摘要: 目的:探讨蛇葡萄素(AMP)对皮离蛋白(DCD)过表达的大肠癌LoVo细胞迁移和侵袭能力的抑制作用。方法:通过质粒瞬时转染使DCD在LoVo细胞中过表达,并以蛋白免疫印迹法检测转染后LoVo细胞中DCD的蛋白表达水平。将细胞分为空载体组、正常对照组、阳性药物组(5-氟尿嘧啶,0.25 μg/mL)和AMP组(300 μmol/L),除空载体组采用转染空载体的LoVo细胞外,其余组均采用转染DCD的LoVo细胞,以Transwell小室迁移实验和划痕实验考察AMP对相应LoVo细胞迁移能力的影响,以Transwell侵袭实验考察AMP对相应LoVo细胞侵袭能力的影响。结果:蛋白免疫印迹法检测结果显示,与空白对照组和空载体组比较,DCD组细胞中DCD的蛋白表达水平均显著升高(P<0.01)。迁移实验和侵袭实验结果显示,与空载体组比较,正常对照组跨膜细胞数均显著增多,细胞迁移率均显著升高(P<0.01);与正常对照组比较,阳性药物组和AMP组跨膜细胞数均显著减少,细胞迁移率均显著降低(P<0.01),且AMP对细胞迁移和侵袭能力的抑制作用均显著优于5-氟尿嘧啶(P<0.01)。结论:DCD过表达能够促进肿瘤细胞迁移和侵袭;AMP对DCD过表达的LoVo细胞的迁移和侵袭能力均具有明显的抑制作用。
ABSTRACT: OBJECTIVE: To investigate the inhibitory effect of ampelopsin(AMP) on the migration and invasion ability of colorectal cancer LoVo cells with dermcidin(DCD) over-expression. METHODS: DCD in LoVo cells was up-regulated by plasmid transient transfection. Western blot method was used to detect the expression of DCD in transfected LoVo cells. Those cells were divided into empty vector group, normal control group, positive drug group (5-fluorouracil, 0.25 μg/mL) and AMP group (300     μmol/L). Except LoVo cells  transfected with empty vector were adopted in empty vector group, LoVo cells transfected with DCD were adopted in other groups. The effects of AMP on migration ability of LoVo cells were studied by Transwell chamber migration and scratch experiments. The invasion ability of LoVo cells was studied by Transwell invasion experiment. RESULTS: Western blot analysis showed that compared with blank control group and empty vector group, the protein expression of DCD in DCD group were increased significantly (P<0.01). Results of migration and invasion experiments showed that compared with empty vector group, the number of transmembrane cells and the rate of cell migration were increased significantly in normal control group (P<0.01). Compared with normal control group, the number of transmembrane cells and the rate of cell migration were decreased significantly in positive drug group and AMP group (P<0.01); inhibitory effects on migration and invasion of AMP were better than 5-fluorouracil (P<0.01). CONCLUSIONS: Over-expressed DCD could promote the migration and invasion of tumor cells.  AMP can significantly inhibit migration and invasion ability of LoVo cells with DCD over-expression.
期刊: 2018年第29卷第22期
作者: 刘丽芳,邱芳华,杨泽填,李秋明
AUTHORS: LIU Lifang,QIU Fanghua,YANG Zetian,LI Qiuming
关键字: 蛇葡萄素;大肠癌;LoVo细胞;皮离蛋白;迁移;侵袭
KEYWORDS: Ampelopsin; Colorectal cancer; LoVo cell; Dermcidin; Migration; Invasion
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