HPLC-DAD法同时测定肝康复水丸剂中7个成分的含量
x

请在关注微信后,向客服人员索取文件

篇名: HPLC-DAD法同时测定肝康复水丸剂中7个成分的含量
TITLE:
摘要: 目的:建立同时测定肝康复水丸剂中没食子酸、栀子苷、芍药内酯苷、芍药苷、丹酚酸B、丹皮酚和丹参酮ⅡA含量的方法。方法:采用高效液相色谱(HPLC)法,以二极管阵列检测器(DAD),色谱柱为Agilent ZORBAX SB-C18;流动相A为乙腈,流动相B为0.1%磷酸溶液,梯度洗脱;流速为1.0 mL/min;检测波长为267 nm(没食子酸)、235 nm(栀子苷、芍药内酯苷、芍药苷)、286 nm(丹酚酸B)、274 nm(丹皮酚)、270 nm(丹参酮ⅡA);柱温为30 ℃;进样量为10 μL。结果:没食子酸、栀子苷、芍药内酯苷、芍药苷、丹酚酸B、丹皮酚、丹参酮ⅡA检测质量浓度的线性范围分别为4.09~51.17 μg/mL(r=0.999 5)、8.35~104.35 μg/mL(r=  0.999 1)、7.78~97.25 μg/mL(r=0.999 9)、7.56~94.54 μg/mL(r=0.999 3)、16.70~208.80 μg/mL(r=0.999 5)、3.46~43.20 μg/mL(r=0.999 7)、23.13~289.10 μg/mL(r=0.999 9),检测限分别为0.09、0.15、0.13、0.12、0.16、0.04、0.24 μg/mL,定量限分别为0.26、0.52、0.48、0.47、0.88、0.18、0.98 μg/mL,平均回收率分别为91.83%、96.11%、96.83%、95.82%、96.93%、93.95%、94.79%(n=6),精密度试验的RSD均≤1.00%(n=6),供试品溶液48 h内稳定性试验的RSD均≤0.96%(n=6),重复性试验的RSD均≤0.99%(n=6)。结论:本方法准确、可靠,可用于肝康复水丸剂中上述7个成分的含量测定。
ABSTRACT: OBJECTIVE: To establish a method for simultaneous determination of the contents of gallic acid, jasminoidin, albiflorin, paeoniflorin, danshinolic acid B, paeonol and tanshinone ⅡA in Gankangfu water pill. METHODS: HPLC method with DAD was adopted. The determination was performed on Agilent ZORBAX SB-C18 column with mobile phase A consisted of acetonitrile and mobile phase B consisted of 0.1% phosphate solution (gradient elution) at the flow rate of 1.0 mL/min. The detection wavelengths were set at 267 nm (gallic acid), 235 nm (jasminoidin, albiflorin, paeoniflorin), 286 nm (danshinolic acid B), 274 nm (paeonol) and 270 nm (tanshinone ⅡA) . The column temperature was set at 30 ℃ and sample size was 10 μL. RESULTS: The linear range of gallic acid, jasminoidin, albiflorin, paeoniflorin, danshinolic acid B, paeonol and tanshinone ⅡA were 4.09-51.17 μg/mL (r=0.999 5), 8.35-104.35 μg/mL (r=0.999 1), 7.78-97.25 μg/mL (r=0.999 9), 7.56-94.54 μg/mL (r=0.999 3), 16.70-208.80 μg/mL (r=0.999 5), 3.46-43.20 μg/mL (r=0.999 7), 23.13-289.10 μg/mL (r=0.999 9), respectively. The detection limits were 0.09, 0.15, 0.13, 0.12, 0.16, 0.04 and 0.24 μg/mL; quantitative limits were 0.26, 0.52, 0.48, 0.47, 0.88, 0.18 and 0.98 μg/mL, respectively. The average recovery rates were 91.83%, 96.11%, 96.83%, 95.82%, 96.93%, 93.95% and 94.79%, respectively (n=6). RSDs of precision test were not more than 1.00% (n=6). RSDs of stability test were not more than 0.96% within 48 h (n=6), and RSDs of repeatability test were than 0.99% (n=6). CONCLUSIONS: The method is accurate and reliable, and can be used for content determination of above 7 components in Gankangfu water pill.
期刊: 2018年第29卷第21期
作者: 侯玉华,袁龙,赵功宝,黄培池,杨慧,曹媛,张洪
AUTHORS: HOU Yuhua,YUAN Long,ZHAO Gongbao,HUANG Peichi,YANG Hui,CAO Yuan,ZHANG Hong
关键字: 高效液相色谱法;二极管阵列检测器;肝康复水丸剂;没食子酸;栀子苷;芍药内酯苷;芍药苷;丹酚酸B;丹皮酚;丹参酮ⅡA;含量测定
KEYWORDS: HPLC; DAD; Gankangfu water pill; Gallic acid; Jasminoidin; Albiflorin; Paeoniflorin; Danshinolic acid B; Paeonol; Tanshinone ⅡA; Content determination
阅读数: 290 次
本月下载数: 7 次

* 注:未经本站明确许可,任何网站不得非法盗链资源下载连接及抄袭本站原创内容资源!在此感谢您的支持与合作!