西伯利亚白刺叶中总生物碱的大孔树脂纯化工艺及抗氧化活性研究
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篇名: 西伯利亚白刺叶中总生物碱的大孔树脂纯化工艺及抗氧化活性研究
TITLE:
摘要: 目的:对西伯利亚白刺叶中总生物碱的大孔树脂纯化工艺进行优化,并评价其抗氧化活性。方法:以样品中总生物碱(以硫酸阿托品计)含量为考察指标,采用静态吸附和解析法对大孔树脂型号进行考察,采用动态吸附和解析法考察上样液质量浓度、上样液pH、上样液流速、柱子径高比、上样量、除杂用水量、洗脱溶剂体积分数,从而对大孔树脂纯化工艺进行优化。以维生素C为阳性对照,考察样品中总生物碱对1,1-二苯基-2-三硝基苯肼(DPPH)自由基的清除能力,以评价其抗氧化活性。结果:HPD-450型大孔树脂对样品中总生物碱的吸附及解析能力较强;最优纯化工艺为上样液质量浓度0.88 mg/mL,上样液pH 6,上样液流速2 BV(柱体积)/h,柱子径高比1 ∶ 8,上样量5 BV,除杂用水量5 BV,洗脱溶剂30%乙醇。按最优纯化工艺优化后的总生物碱含量为16.94 mg/g,半数抑制浓度为(58.78±3.00)μg/mL。结论:优化的纯化工艺稳定、可行,可较好地分离和纯化西伯利亚白刺叶中的总生物碱,且纯化得到的总生物碱具有一定的抗氧化活性,其中30%乙醇洗脱得到的总生物碱清除DPPH自由基的能力最强。
ABSTRACT: OBJECTIVE: To optimize the purification technology of total alkaloids from the leaves of Nitraria sibirica with macroporous resin, and to evaluate its antioxidant activity. METHODS: Using the content of total alkaloids (by atropine sulfate) as index, static adsorption and analytical method were used to investigate the type of macroporous resin. Dynamic adsorption and analytical method were used to investigate the concentration of the sample solution, the pH of the sample solution, the flow rate of the sample solution, diameter-height ratio of column, sample capacity, the volume of elution water and volume fraction of elution solvent so as to optimize macroporous resin purification technology. Using vitamin C as positive control, scavenging capacity of total alkaloids to 1, 1- diphenyl-2-diphenyl picryl hydrazinyl (DPPH) radicals was investigated to evaluate its antioxidant activity. RESULTS: The adsorption and desorption effects of HPD-450 macroporous resin on total alkaloids were the best. The optimal purification technology included the sample solution concentration 0.88 mg/mL, sample solution pH 6, sample solution flow rate 2 BV/h,diameter-height ratio of column 1 ∶ 8, sample capacity 5 BV, the volume of elution water 5 BV, elution solvent 30% ethanol. The content of total alkaloid was 16.94 mg/g, and IC50 was (58.78±3.00)μg/mL by optimal purification technology. CONCLUSIONS: Optimized purification technology is stable and feasible, and can separate and purify total alkaloids from the leaves of N. sibirica. Purified total alkaloids display good antioxidant activities. The total alkaloids eluted with 30% ethanol show the strongest scavenging activity to DPPH radicals.
期刊: 2018年第29卷第20期
作者: 其曼古丽·吐尔洪,玉苏普·买买提,马依努尔·拜克力
AUTHORS: Chimengul·TURGHUN,Yusup·MAMAT,Mahinur·BAKRI
关键字: 西伯利亚白刺叶;总生物碱;大孔树脂;纯化工艺;抗氧化活性
KEYWORDS: Leaves of Nitraria sibirica; Total alkaloids; Macroporous resin; Purification technology; Antioxidant activity
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