莪红片对急性血瘀证模型大鼠血液流变学及血栓形成相关指标的影响
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篇名: 莪红片对急性血瘀证模型大鼠血液流变学及血栓形成相关指标的影响
TITLE:
摘要: 目的:观察莪红片对急性血瘀证模型大鼠血液流变学及血栓形成相关指标的影响。方法:将72只雄性SD大鼠随机分为空白组、模型组、脑心通胶囊组(0.8 g/kg)和莪红片低、中、高剂量组(0.375、0.75、1.5 g/kg),每组12只。各给药组大鼠均灌胃相应药物,空白组和模型组大鼠灌胃等容生理盐水,每天1次,连续7 d。除空白组外,其余各组大鼠均于末次给药1 h后皮下注射盐酸肾上腺素(0.06 mg/kg),2 h后用冷水刺激3 min,并于2 h后再次皮下注射同等剂量的盐酸肾上腺素以复制急性血瘀证模型。禁食、不禁水20 h后,观察各组大鼠行为,并检测其全血黏度(高、中、低切)和血清血栓素B2(TXB2)、6-酮-前列腺素F1a(6-k-PGF1a)含量。结果:空白组大鼠饮食正常,精神和活动状态良好;模型组大鼠出现食欲不振、精神萎靡、活动减少等症状,其全血黏度(高、中、低切)和血清TXB2含量均较空白组显著升高,6-k-PGF1a含量较空白组显著降低,差异均有统计学意义(P<0.01);各给药组大鼠上述症状均有不同程度改善,且脑心通胶囊组和莪红片中、高剂量组大鼠全血黏度(高、中、低切)和血清TXB2含量均较模型组显著降低,6-k-PGF1a含量均较模型组显著升高,差异均有统计学意义(P<0.05或P<0.01)。结论:莪红片可一定程度地降低全血黏度、改善血瘀症状,这种作用可能与其下调血清TXB2、上调6-k-PGF1a含量有关。
ABSTRACT: OBJECTIVE: To observe the effects of Ehong tablets on hemorheology and related indexes of thrombosis in acute blood stasis model rats. METHODS: Totally 72 male SD rats were randomly divided into blank group, model group, Naoxintong capsules group (0.8 g/kg) and Ehong tablets low-dose, medium-dose and high-dose groups (0.375, 0.75, 1.5 g/kg), with 12 rats in each group. Administration groups were given relevant medicine intragastrically; blank group and model group were given constant volume of normal saline intragastrically, once a day, for consecutive 7 d. Except for blank group, other groups were given adrenaline hydrochloride (0.06 mg/kg) subcutaneously 1 h after last medication, 2 h later were irritated with cold water for 3 min, and then given same dose of adrenaline hydrochloride subcutaneously again 2 h later to induce acute blood stasis model. Twenty hours after fasting without water fasting, the behaviors of rats were observed, whole blood viscosity (high, medium and low shear) and serum contents of TXB2 and 6-k-PGF1a were detected. RESULTS: The rats in the normal group had normal diet, good mental and active state. The model group suffered from loss of appetite, depression and activity decreasing, etc.; whole blood viscosity (high, medium and low shear) and serum contents of TXB2 were increased significantly, while serum content of 6-k-PGF1a was decreased significantly, compared with blank group, with statistical significance (P<0.01). Compared with model group, above symptoms of rats were improved to different extents in administration groups. The whole blood viscosity (high, medium and low shear) and serum contents of TXB2 were decreased significantly in Naoxintong capsules group, Ehong tablets medium-dose and high-dose groups, while serum contents of 6-k-PGF1a was increased significantly, with statistical significance (P<0.05 or P<0.01). CONCLUSIONS: Ehong tablets can reduce blood viscosity and improve blood stasis symptoms to certain extent, the effects of which may be associated with down-regulating serum content of TXB2 and up-regulating serum content of 6-k-PGF1a.
期刊: 2018年第29卷第18期
作者: 郝少君,谢国旗,李重先,李军,刘晓斌,李文俊,张正臣
AUTHORS: HAO Shaojun,XIE Guoqi,LI Chongxian,LI Jun,LIU Xiaobin,LI Wenjun,ZHANG Zhengchen
关键字: 莪红片;大鼠;急性血瘀证模型;全血黏度;血栓素B2;6-酮-前列腺素F1a
KEYWORDS: Ehong tablets; Rats; Acute blood stasis model; Blood viscosity; TXB2; 6-k-PGF1a
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