紫苏叶总三萜超声提取工艺优化及对10种常见致病菌的抑菌作用研究
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篇名: 紫苏叶总三萜超声提取工艺优化及对10种常见致病菌的抑菌作用研究
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摘要: 目的:优化紫苏叶总三萜的超声提取工艺,并考察其对10种常见致病菌的抑菌作用。方法:以紫苏叶总三萜提取量为响应值,以料液比、乙醇体积分数、超声时间、超声温度为响应因子,在单因素试验的基础上,采用响应面法优化紫苏叶总三萜的超声提取工艺;采用微量二倍稀释法测定紫苏叶总三萜对10种常见致病菌的最低抑菌浓度(MIC)和最低杀菌浓度(MBC),以考察其抑菌作用。结果:优化的紫苏叶总三萜超声提取工艺为料液比1 ∶ 30[m(g)/V(mL)],乙醇体积分数85%,超声时间65 min,超声温度75 ℃。在此条件下的紫苏叶总三萜提取量为37.90 mg/g,与预测值(38.40 mg/g)的相对误差为1.30%,实测值与预测值一致性良好。抑菌作用考察结果表明,紫苏叶总三萜对10种常见致病菌的MIC范围为0.48~15.50 mg/mL,MBC≥0.97 mg/mL,整体抑菌作用较强,尤其对大肠杆菌、金黄色葡萄球菌和铜绿假单胞菌的抑菌作用最强。结论:优化所得紫苏叶总三萜的超声提取工艺稳定、可行;紫苏叶总三萜具有较强的广谱抗菌作用,且对大肠杆菌、金黄色葡萄球菌、铜绿假单胞菌的抗菌作用相当。
ABSTRACT: OBJECTIVE: To optimize the ultrasonic extraction technology of total triterpenoids from the leaves of Perilla frutescens (TPL) and investigate its antibacterial activities against 10 kinds of common pathogens. METHODS: Using the extraction amount of triterpenoids as response value, the ultrasonic extraction technology of TPL was optimized by response surface methodology on the basis of single factor test with solid-to-liquid ratio, ethanol volume fraction, ultrasonic time and temperature as factors. The MIC and MBC of TPL on 10 kinds of common pathogens were detected by double broth dilution method, so as to investigate antibacterial activities. RESULTS: The optimal ultrasonic extraction technology of TPL included solid-to-liquid ratio of 1 ∶ 30 [m(g)/V(mL)], ethanol volume fraction of 85 %, ultrasonic time of 65 min and ultrasonic temperature of 75 ℃. Under these conditions, the amount of triterpenoids was 37.90 mg/g, relative error of which to predicted value (38.40 mg/g) was 1.30%. Measured value agreed well with predicted value. Results of antibacterial activities study showed that MIC were in the range of 0.48-15.50 mg/mL,and MBC≥0.97 mg/mL. TPL showed significant antibacterial activities, especially to Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa. CONCLUSIONS: Optimized ultrasonic extraction technology of TPL is stable and feasible.  TPL extracted has strong broad-spectrum antibacterial effect and shows similar antibacterial effecton E.coli, S. anreus and P. aeruginosa.  
期刊: 2018年第29卷第16期
作者: 魏磊,李晓,王学方,景炳年,陈飞,崔炜,郭唯,杜美,王伟
AUTHORS: WEI Lei,LI Xiao,WANG Xuefang,JING Bingnian,CHEN Fei,CUI Wei,GUO Wei,DU Mei,WANG Wei
关键字: 紫苏叶;总三萜;超声提取工艺;响应面法;致病菌;抑菌作用;微量二倍稀释法
KEYWORDS: Perilla frutescens; Total triterpenoids; Ultrasonic extraction technology; Response surface methodology; Pathogens; Antibacterial activities; Double broth dilution method
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