基于系统药理学的茵陈作用机制研究
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篇名: 基于系统药理学的茵陈作用机制研究
TITLE:
摘要: 目的:探讨茵陈可能的药理作用机制,为其进一步开发利用提供参考。方法:利用中药系统药理学分析平台数据库(TCMSP)筛选茵陈的有效成分和相关靶标蛋白;运用Cytoscape 3.5.1软件构建茵陈有效化合物-靶标蛋白可视化网络,并进行拓扑学分析;借助STRING数据库进行蛋白质与蛋白质相互作用(PPI)网络的构建与分析;通过DAVID生物信息学资源数据库对靶标蛋白编码基因进行KEGG通路富集分析。结果:共筛选出茵陈有效化合物13个,靶标蛋白189个,KEGG富集通路34条。槲皮素、β-谷甾醇、异鼠李素、Artepillin C等是主要有效化合物,前列腺素G/H合成酶2(PTGS2)、热休克蛋白90(HSP90)、二肽基肽酶Ⅳ、蛋白激酶A催化亚基Cα等是主要靶标蛋白;转录因子AP-1(JUN)、细胞肿瘤抗原p53在PPI网络中发挥了关键作用;靶标蛋白编码基因主要富集在肿瘤坏死因子α(TNF-α)信号通路、缺氧诱导因子1(HIF-1)信号通路、Toll样受体信号通路、磷脂酰肌醇-3-激酶/丝苏氨酸蛋白激酶(P13K/Akt)信号通路、T细胞受体信号通路、甲状腺激素信号通路、凋亡信号通路等通路上。结论:茵陈中的槲皮素、β-谷甾醇、异鼠李素等化合物可能通过TNF-α信号通路、HIF-1信号通路、PI3K/Akt信号通路等作用于PTGS2、HSP90、JUN等靶标蛋白,进而发挥其抗炎、抗肿瘤等药理作用。
ABSTRACT: OBJECTIVE: To investigate the possible mechanism of Artemisia capillaries, and to provide reference for further development and utilization of it. METHODS: The effective components and related target protein of A. capillaries were screened by Traditional Chinese Medicine Systems Pharmacology (TCMSP) analysis platform database. The effective compound-target protein visual network of A. capillaries was established by using Cytoscape 3.5.1 software, topology analysis was also performed. The protein-protein interaction (PPI) network was constructed and analyzed by STRING database. KEGG pathway enrichment of target protein coding gene was analyzed by DAVID bioinformatics resource database. RESULTS: A total of 13 kinds of effective compounds, 189 target proteins and 34 enrichment pathways were selected. Quercetin, β-glutamol, isorhamnetin and artepillin C were main effective compounds. Prostaglandin G/H sythase 2(PTGS 2), heat shock protein 90(HSP 90), dipeptidyl peptidase Ⅳ, protein kinase A catalytic subunit Cα were main target proteins. Transcription factor AP-1 and cell tumor antigen p53 played a key role in PPI network. The target protein coding gene was rich in TNF-α signaling pathway, HIF-1 signaling pathway, Toll-like receptor signaling pathway, PI3K/Akt signaling pathway, T cell receptor signaling pathway, thyroid hormone signaling pathway, apoptotic signaling pathway, etc. CONCLUSIONS: Quercetin, β-glutamol and isorhamnetin of A. capillaries play an effect on PTGS2, HSP90, transcription factor AP-1 and other target proteins through TNF-α signaling pathway, HIF-1 signaling pathway and PI3K/Akt signaling pathway, so as to play anti-inflammatory and antitumor effect.
期刊: 2018年第29卷第10期
作者: 陈国铭,汤顺莉,邝梓君,黄雁,赵金龙,於菁雯,陈子茵,林洪荣,黄楚瑶,许华
AUTHORS: CHEN Guoming,TANG Shunli,KUANG Zijun,HUANG Yan,ZHAO Jinlong,YU Jingwen,CHEN Ziyin,LIN Hongrong,HUANG Chuyao,XU Hua
关键字: 系统药理学;茵陈;有效化合物;靶标蛋白;信号通路;药理作用
KEYWORDS: Systems pharmacology; Artemisia capillaries; Effective compound; Target protein; Signaling pathway; Pharmacological effect
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