无梗五加果实3种提取物对人肝癌细胞SMMC-7721增殖和凋亡的影响
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篇名: 无梗五加果实3种提取物对人肝癌细胞SMMC-7721增殖和凋亡的影响
TITLE:
摘要: 目的:研究无梗五加果实3种提取物对人肝癌细胞SMMC-7721增殖、细胞周期和凋亡的影响,为明确其抗肿瘤作用机制提供参考。方法:采用MTT法考察低、中、高质量浓度的无梗五加果实乙醇提取物(0.92、1.84、3.68 mg/mL)、粗多糖提取物(0.06、0.12、0.24 mg/mL)和精多糖提取物(0.04、0.08、0.16 mg/mL)分别作用24、36、48 h后对人肝癌细胞SMMC-7721的增殖抑制作用;采用流式细胞术考察1.84 mg/mL乙醇提取物、0.24 mg/mL粗多糖提取物和0.16 mg/mL精多糖提取物分别作用24 h后对人肝癌细胞SMMC-7721细胞周期和细胞凋亡的影响。以上试验均设阴性对照(只加细胞不加药物)。结果:与阴性对照比较,无梗五加果实3种提取物均可显著抑制人肝癌细胞SMMC-7721的增殖(P<0.01),显著降低G0/G1、G2/M期细胞的百分率(P<0.01),显著升高S期细胞的百分率(P<0.01),显著升高细胞凋亡率(P<0.05),其中以无梗五加果实乙醇提取物的作用最为明显。结论:无梗五加果实3种提取物均能抑制人肝癌细胞SMMC-7721的增殖,阻滞其细胞周期于S期,诱导其凋亡。
ABSTRACT: OBJECTIVE: To study the effects of 3 extracts of Acanthopanax sessiliflorus fruits on the proliferation and apoptosis of human hepatocarcinoma cells SMMC-7721, and to provide reference for confirming the mechanism of anti-tumor effect. METHODS: MTT assay was adopted to investigate the effects of low-mass concentration, medium-mass concentration and high-mass concentration of ethanol extract (0.92, 1.84, 3.68 mg/mL), crude polysaccharide extract (0.06, 0.12, 0.24 mg/mL) and refined polysaccharide extract (0.04, 0.08, 0.16 mg/mL) from A. sessiliflorus fruits on the proliferation and apoptosis of SMMC-7721 cells after treated for 24, 36, 48 h, respectively. Flow cytometry was used to investigate the effects of 1.84 mg/mL ethanol extract, 0.24 mg/mL crude polysaccharide extract and 0.16 mg/mL refined polysaccharide extract on cell cycle and cell apoptosis after treated for 24 h. The above tests were all negative control (only adding cells without drugs). RESULTS: Compared with negative control, 3 extracts of A. sessiliflorus fruits could significantly inhibit the proliferation of SMMC-7721 cells (P<0.01), could significantly decrease the percentage of SMMC-7721 cells in G0/G1 and G2/M phase (P<0.01), could significantly increase the percentage of SMMC-7721 cells in S phase (P<0.01) and the apoptosis rate of SMMC-7721 cells (P<0.05); especially the effects of ethanol extract from A. sessiliflorus fruits were the most obvious. CONCLUSIONS: Three extracts of A. sessiliflorus fruits can inhibit the proliferation of human hepatocarcinoma SMMC-7721 cells, block SMMC-7721 cells in S phase and induce the apoptosis of SMMC-7721 cells.
期刊: 2018年第29卷第9期
作者: 刘玉强,管美玉,孙建之,才谦
AUTHORS: LIU Yuqiang,GUAN Meiyu,SUN Jianzhi,CAI Qian
关键字: 无梗五加果实;乙醇提取物;粗多糖提取物;精多糖提取物;人肝癌细胞SMMC-7721;增殖;凋亡
KEYWORDS: Acanthopanax sessiliflorus fruits; Ethanol extract; Crude polysaccharide extract; Refined polysaccharide extract; Human hepatocarcinoma cells SMMC-7721; Proliferation; Apoptosis
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