不同栽培年限川桔梗中桔梗总皂苷和桔梗皂苷D的含量比较
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篇名: 不同栽培年限川桔梗中桔梗总皂苷和桔梗皂苷D的含量比较
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摘要: 目的:建立测定川桔梗中桔梗总皂苷及桔梗皂苷D含量的方法,探索不同栽培年限川桔梗药材中2种指标成分的差异。方法:采用紫外-可见分光光度法测定川桔梗中桔梗总皂苷的含量;采用高效液相色谱法测定川桔梗中桔梗皂苷D的含量;比较一年生、两年生及三年生各10份川桔梗药材中桔梗总皂苷及桔梗皂苷D的含量。结果:一年生、二年生、三年生川桔梗药材中桔梗总皂苷含量的平均值分别为2.47%、3.01%、2.47%;桔梗皂苷D含量的平均值分别为0.23%、0.27%、0.33%;即一年生的样品中2种指标的含量均较低,二年生样品的桔梗总皂苷含量最高,三年生样品桔梗皂苷D的含量最高。结论:不同栽培年限的川桔梗药材的指标性成分含量具有一定差异,但二者间并未呈现相关性,建议选用栽培年限为二年生或三年生的药材。
ABSTRACT: OBJECTIVE: To establish a method for content determination of total saponins and platycodin D in Platycodon grandiflorum from Sichuan and investigate the difference of 2 indexes in P. grandiflorum from Sichuan of different cultivated years. METHODS: The content of total saponins in P. grandiflorum from Sichuan was determined by UV spectrophotometry. The content of platycodin D was determined by HPLC. The contents of total saponins and platycodin D were compared among each 10 samples of annual, biennial and triennial P. grandiflorum from Sichuan. RESULTS: Average contents of total saponins in annual, biennial and triennial P. grandiflorum from Sichuan were 2.47%, 3.01%, 2.47%, respectively; average contents of platycodin D were 0.23%, 0.27%, 0.33%, respectively. The contents of 2 indexes in annual P. grandiflorum from Sichuan were in relative low level, while the content total saponins in biennial P. grandiflorum from Sichuan was the highest; the content of platycodin D in triennial P. grandiflorum was the highest. CONCLUSIONS: The contents of indexes are different among P. grandiflorum from Sichuan of different cultivated years. But there is no correlation between them. It is suggested to select biennial and triennial P. grandiflorum from Sichuan.
期刊: 2018年第29卷第9期
作者: 李心怡,陈玲,马逾英,邓薇
AUTHORS: LI Xinyi,CHEN Ling,MA Yuying,DENG Wei
关键字: 川桔梗;桔梗总皂苷;桔梗皂苷D;栽培年限;含量测定
KEYWORDS: Platycodon grandiflorum from Sichuan; Total saponins; Platycodin D; Cultivated years; Content determination
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