新仙灵骨葆胶囊对大鼠骨折愈合的影响研究
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篇名: 新仙灵骨葆胶囊对大鼠骨折愈合的影响研究
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摘要: 目的:研究新仙灵骨葆胶囊对大鼠骨折愈合的影响,为寻求“减量增效(减量等效)”新组方提供参考。方法:将大鼠随机分为假手术组(蒸馏水)、模型组(蒸馏水)、仙灵骨葆胶囊组(350 mg/kg)和新仙灵骨葆胶囊低、中、高剂量组(53、105、210 mg/kg),每组14只。除假手术组外,其余各组大鼠均在麻醉状态下制作右侧股骨中段骨折模型。待大鼠术后清醒,各组大鼠灌胃相应药物10 mL/kg,每天给药1次,连续4周。末次给药后,测定大鼠体质量,观察骨折处骨痂形成及组织病理学变化,进行骨折侧生物力学(骨折应力、骨压碎力)测量,并采用酶联免疫吸附法检测血清中炎症因子[肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)] 水平。结果:与假手术组比较,模型组大鼠体质量和骨折侧骨折应力、骨压碎力明显降低(P<0.05或P<0.01),血清中TNF-α、IL-1β水平明显升高(P<0.01);模型组大鼠骨折周围可见明显骨痂形成,骨小梁不成熟、排列混乱。与模型组比较,新仙灵骨葆胶囊高剂量组和仙灵骨葆胶囊组大鼠体质量明显增加(P<0.05),新仙灵骨葆胶囊中、高剂量组和仙灵骨葆胶囊组大鼠骨折侧骨折应力明显升高(P<0.05或P<0.01),各给药组大鼠血清中TNF-α、IL-1β水平均明显降低(P<0.01);且新仙灵骨葆胶囊各剂量组与仙灵骨葆胶囊组比较上述指标差异均无统计学意义(P>0.05)。各给药组大鼠骨痂面积均变小,骨折线变模糊,骨折痕迹基本消失,骨小梁数量增多;骨折处皮质层增厚,骨髓腔见大量毛细血管植入。结论:新仙灵骨葆胶囊具有明显的促进骨折愈合作用,可有效提高骨生物力学强度,抑制炎症反应。
ABSTRACT: OBJECTIVE: To study the effects of new Xianling gubao capsule on fracture healing in rats, and to provide reference for seeking new formula of “to reduce stockpiles and increase efficiency (to reduce stockpiles and constant efficiency)”. METHODS: Rats were randomly divided into sham operation group (distilled water), model group (distilled water), Xianling gubao capsule group (350 mg/kg), new Xianling gubao capsule low-dose, medium-dose and high-dose groups (53, 105, 210    mg/kg), with 14 rats in each group. Except for sham operation group, right middle femur fracture model was established under the anesthetic state. Rats were given relevant medicine 10 mL/kg intragastrically after woken, once a day, for consecutive 4 weeks. After last administration, body weights of rats were determined; the formation of callus and histopathological changes in fracture were observed; biomechanics of fracture side (fracture stress and bone crushing force) was measured. The levels of inflammatory factors (TNF-α, IL-1β) in serum were detected by ELISA. RESULTS: Compared with sham operation group, body weight, fracture stress and bone crushing force of fracture side were decreased significantly (P<0.05 or P<0.01), while the serum levels of TNF-α and IL-1β were increased significantly (P<0.01). In model group, the scab was visible around the fracture, and the trabecular bone was not mature and arranged in confusion. Compared with model group, body weights of rats were increased significantly in new Xianling gubao capsule high-dose group and Xianling gubao capsule group (P<0.05); fracture stress of fracture side were increased significantly in new Xianling gubao capsule medium-dose and high-dose groups and Xianling gubao capsule group (P<0.05 or P<0.01). The serum levels of TNF-α and IL-1β were decreased significantly (P<0.01). There was no statistical significance in above indexes among new Xianling gubao capsule groups and Xianling gubao capsule group (P>0.05). The area of the callus in each group was smaller; the fracture line became blurred; the fracture trace disappeared and the number of bone trabeculae increased. The cortical layer was thickened, and a large number of capillary implants were found in the bone marrow cavity. CONCLUSIONS: New Xianling gubao capsule has a significant role in promoting fracture healing, and can effectively improve the strength of bone biomechanics and inhibit the inflammatory reaction.
期刊: 2018年第29卷第9期
作者: 刘明,刘杨,张永萍,徐剑,孙宜春,白志文
AUTHORS: LIU Ming,LIU Yang,ZHANG Yongping,XU Jian,SUN Yichun,BAI Zhiwen
关键字: 新仙灵骨葆胶囊;大鼠;骨折;骨生物力学;炎症因子
KEYWORDS: New Xianling gubao capsule; Rats; Fracture; Bone biomechanics; Inflammatory factors
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