辣蓼提取物对大鼠急性胃黏膜损伤的保护作用研究
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篇名: 辣蓼提取物对大鼠急性胃黏膜损伤的保护作用研究
TITLE:
摘要: 目的:研究辣蓼提取物对大鼠急性胃黏膜损伤(AGML)的保护作用。方法:将48只大鼠随机分为正常组(生理盐水)、模型组(生理盐水)、阳性组(盐酸雷尼替丁,0.05 g/kg)和辣蓼提取物低、中、高剂量组(以生药量计分别为2.7、8.1、24.3 g/kg),连续灌胃给药7 d,每天1次。末次给药1 h后,除正常组外,其余各组大鼠均灌胃无水乙醇复制AGML模型。造模1.5 h后,计算各组大鼠胃黏膜损伤指数,观察大鼠胃组织病理学变化,采用酶联免疫吸附法测定大鼠胃组织中核因子E2相关因子2(Nrf2)含量和超氧化物歧化酶(SOD)活性。结果:与正常组比较,模型组大鼠胃黏膜损伤明显,黏膜下层毛细血管破裂出血,胃黏膜损伤指数显著升高(P<0.01);胃组织中 Nrf2含量和SOD 活性显著降低(P<0.05或P<0.01)。与模型组比较,各给药组大鼠胃黏膜的损伤均不同程度减轻;阳性组和辣蓼提取物中、高剂量组大鼠胃黏膜损伤指数显著降低(P<0.05),胃组织中Nrf2含量和SOD 活性显著升高(P<0.05或P<0.01)。结论:辣蓼提取物对无水乙醇致大鼠AGML具有较好的保护作用,其机制可能与提高胃黏膜组织中Nrf2含量和增强SOD 活性有关。
ABSTRACT: OBJECTIVE: To study the protective effects of Polygonum hydropiper extract on acute gastric mucosal lesion (AGML) in rats. METHODS: 48 rats were randomly divided into normal group (normal saline), model group (normal saline), positive group (ranitidine hydrochloride, 0.05 g/kg), P. hydropiper extract low-dose, medium-dose and high-dose groups (2.7, 8.1, 24.3 g/kg by crude drug), i.g. for consecutive 7 d, once a day. Except for normal group, other groups were given absolute ethyl alcohol to induce AGMI model after 1 h of last administration. 1.5 h after modeling, gastric mucosal lesion index of rats was calculated; the pathological changes of gastric tissue in rats were observed; nuclear factor E2 related factor 2 (Nrf2) content and SOD activity in gastric tissue of rats were determined by ELISA. RESULTS: Compared with normal group, the gastric mucosa of model group was damaged obviously, there was blood capillary rupture in submucosa, gastric mucosal lesion index was increased significantly(P<0.01); Nrf2 content and SOD activity were significantly decreased in gastric tissue of rats (P<0.05 or P<0.01). Compared with model group, gastric mucosal lesion of rats was relieved to different extent; in positive group, P. hydropiper extract medium-dose and high-dose groups, gastric mucosal lesion index was decreased significantly (P<0.05), and Nrf2 content and SOD activity were increased significantly(P<0.05 or P<0.01). CONCLUSIONS: P. hydropiper extract has good protective effect on absolute ethyl alcohol-induce AGMI, the mechanism of which may be associated with raising Nrf2 content and enhancing SOD activity in gastric mucosal tissue.
期刊: 2018年第29卷第7期
作者: 任守忠,苏文琴,朱宏锐,王宁,牛海艳,赵雅媚,马志健
AUTHORS: REN Shouzhong,SU Wenqin,ZHU Hongrui,WANG Ning,NIU Haiyan,ZHAO Yamei,MA Zhijian
关键字: 辣蓼提取物;急性胃黏膜损伤;核因子E2相关因子2;超氧化物歧化酶;大鼠
KEYWORDS: Polygonum hydropiper extract; Acute gastric mucosal lesion; Nuclear factor E2 related factor 2; SOD; Rats
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