超微粉碎技术对灵芝中三萜类成分溶出的影响
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篇名: 超微粉碎技术对灵芝中三萜类成分溶出的影响
TITLE:
摘要: 目的:研究超微粉碎技术对灵芝中三萜类成分溶出的影响。方法:采用紫外-可见分光光度法测定灵芝中三萜类成分总提取率,高效液相色谱法(HPLC)测定灵芝中9种三萜类成分(灵芝酸A、灵芝酸B、灵芝酸C、灵芝酸C1、灵芝酸C2、灵芝酸D、灵芝酸E、灵芝酸G、灵芝酸H)含量,比较30、50、80目灵芝普通粉和300目灵芝超微粉中三萜类成分总提取率及9种三萜类成分的含量。结果:30、50、80、300目灵芝粉中三萜类成分总提取率分别为(0.74±0.08)%、(0.75±0.06)%、(0.78±0.06)%、(1.09±0.10)%(RSD<2%,n=3);随着灵芝粉目数的增大,三萜类成分含量逐渐增大,其中300目含量最大,显著高于灵芝普通粉(P<0.05)。结论:灵芝经超微粉碎后三萜类成分的溶出增加。
ABSTRACT: OBJECTIVE: To study the effects of ultrafine grinding technology on the dissolution of triterpenoids from Ganoderma lucidum. METHODS: UV spectrophotometry was used to determine total extration rates of triterpenoids from G. lucidum. HPLC method was used to determine the contents of 9 kinds of triterpenoids (ganoderic acid A, ganoderic acid B, ganoderic acid C, ganoderic acid C1, ganoderic acid C2, ganoderic acid D, ganoderic acid E, ganoderic acid G, ganoderic acid H) from G. lucidum. The total extration rates and the contents of 9 kinds of triterpenoids were compared between 30, 50, 80 mesh common G. lucidum powder and 300 mesh G. lucidum ultramicro powder. RESULTS: The total extraction rates of triterpenoids from 30, 50, 80, 300 mesh G. lucidum powder were (0.74±0.08)%, (0.75±0.06)%, (0.78±0.06)%, (1.09±0.10)% (RSD<2%,n=3), respectively. With the increase of the mesh number of G. lucidum powder, the contents of triterpenoids were increased gradually, and were the highest in 300 mesh, which was significant higher than common G. lucidum powder (P<0.05). CONCLUSIONS: After ultrafine grinding, the dissolution of triterpenoids from G. lucidum is increased.
期刊: 2018年第29卷第5期
作者: 张福君,瞿晶田,王强
AUTHORS: ZHANG Fujun,QU Jingtian,WANG Qiang
关键字: 灵芝;超微粉碎技术;三萜类成分;高效液相色谱法
KEYWORDS: Ganoderma lucidum; Ultrafine grinding technology; Triterpenoids; HPLC
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