RP-HPLC法同时测定新疆紫草中紫草素、乙酰紫草素、β,β-二甲基丙烯酰紫草素的含量
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篇名: RP-HPLC法同时测定新疆紫草中紫草素、乙酰紫草素、β,β-二甲基丙烯酰紫草素的含量
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摘要: 目的:建立同时测定新疆紫草中紫草素、乙酰紫草素、β,β-二甲基丙烯酰紫草素含量的方法。方法:采用反相高效液相色谱法,色谱柱为Kromasil 100-5 C18,流动相为乙腈-0.1%甲酸溶液(80 ∶ 20,V/V),流速为1.0 mL/min,检测波长为516 nm,柱温为25 ℃,进样量为10 μL。结果:紫草素、乙酰紫草素、β,β-二甲基丙烯酰紫草素检测质量浓度线性范围分别为0.404~10.100 μg/mL(r=0.999 8)、5.350~107.000 μg/mL(r=0.999 6)、2.035~40.700 μg/mL(r=0.999 8);定量限分别为0.40、2.91、1.34 μg/mL,检测限分别为0.12、0.87、0.40 μg/mL;精密度、稳定性、重复性试验的RSD<2.0%(n=6);加样回收率分别为99.12%~104.18%(RSD=1.85%,n=6)、96.51%~100.21%(RSD=1.43%,n=6)、98.11%~102.51%(RSD=1.42%,n=6)。结论:该方法操作简便,精密度、稳定性、重复性好,可用于新疆紫草中紫草素、乙酰紫草素、β,β-二甲基丙烯酰紫草素含量的同时测定。
ABSTRACT: OBJECTIVE: To establish a method for the simultaneous determination of shikonin, acetylshikonin and β,β-dimethylacrylshikonin in Arnebia euchroma. METHODS: RP-HPLC method was adopted. The determination was performed on Kromasil 100-5 C18 column with mobile phase consisted of acetonitrile-0.1% formic acid solution(80 ∶ 20,V/V) at the flow rate of 1.0 mL/min. The detection wavelength was set at 516 nm, column temperature was 25 ℃, and sample size was 10 μL. RESULTS: The linear ranges of shikonin, acetylshikonin and β,β-dimethylacrylshikonin were 0.404-10.100 μg/mL(r=0.999 8), 5.350-107.000 μg/mL(r=0.999 6), 2.035-40.700 μg/mL(r=0.999 8), respectively. The limit of quantitation was 0.40,2.91,1.34 μg/mL, and the limit of detection was 0.12,0.87,0.40 μg/mL. RSDs of precision, stability and reproducibility tests were all lower than 2.0% (n=6). The recovery rate were 99.12%-104.18%(RSD=1.85%,n=6),96.51%-100.21%(RSD=1.43%,n=6), 98.11%-102.51% (RSD=1.42%,n=6), respectively. CONCLUSIONS: The method is simple, precise, stable and reproducible. It can be used for simultaneous determination of shikonin, acetylshikonin and β,β-dimethylacrylshikonin in A. euchroma.
期刊: 2018年第29卷第4期
作者: 孙健文,温宪春,蔡德富,王玥,吴永会
AUTHORS: SUN Jianwen,WEN Xianchun,CAI Defu,WANG Yue,WU Yonghui
关键字: 紫草;紫草素;乙酰紫草素;β,β-二甲基丙烯酰紫草素;反相高效液相色谱法;含量
KEYWORDS: Arnebia euchroma; Shikonin; Acetylshikonin; β,β-dimethylacrylshikonin; RP-HPLC;Content
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