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10批不同产地六棱菊中咖啡酸的工艺优化与含量测定
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| 篇名： | 10批不同产地六棱菊中咖啡酸的工艺优化与含量测定 |
| TITLE： | |
| 摘要： | 目的：优化六棱菊中咖啡酸提取工艺，并建立其含量测定方法。方法：以回流提取法提取六棱菊中咖啡酸。以提取含量为考察指标，设计正交试验，考察乙醇体积分数、料液比及提取时间对咖啡酸提取的影响，优化提取工艺条件。采用高效液相色谱法，以咖啡酸为对照品，在320 nm波长下测定10批不同产地六棱菊药材中的咖啡酸含量。结果：优化的提取工艺条件为乙醇体积分数为10％、料液比为1 ∶ 40、提取时间为3 h。以此条件对咖啡酸进行工艺验证试验，六棱菊中咖啡酸平均含量为0.521 1 mg/g（RSD＝1.18％，n＝3）。10批不同产地六棱菊药材中咖啡酸含量在0.375 2～0.776 6 mg/g之间，含量差异较大。结论：六棱菊药材中咖啡酸含量与产地和采收季节等有关。本试验优选工艺提取咖啡酸重复性较好；建立的咖啡酸含量测定方法稳定、可行。 |
| ABSTRACT： | OBJECTIVE： To optimize the extraction technology of caffeic acid in Laggera alata， and establish a method for its content determination. METHODS： The caffeic acid in L. alata was extracted by reflux extraction. Using extraction content as investigation index， orthogonal test was used to investigate the effects of ethanol volume fraction， material-liquid ratio and extraction time on caffeic acid， and the extraction technology conditions were optimized. HPLC was adopted to determine the content of caffeic acid in 10 batches of L. alata from different areas， using caffeic acid as reference substance， at wavelength of 320 nm. RESULTS： The optimized extraction technology conditions were as follows as ethanol volume fraction of 10％， material-liquid ratio of 1 ∶ 40 and extraction time of 3 h. Under the condition， verification test for caffeic acid was carried out， and the average content of caffeic acid in L. alata was 0.521 1 mg/g （RSD＝1.18％， n＝3）. The content of caffeic acid in 10 batches of L. alata from different areas ranged in 0.375 2-0.776 6 mg/g， and the content showed great differences. CONCLUSIONS： The content of caffeic acid in L. alata is related to area and harvest season. The caffeic acid extration by optimized technology shows good reproducibility； and the established method for content determination is stable and feasible. |
| 期刊： | 2017年第28卷第34期 |
| 作者： | 魏江存，陈勇，谢臻，李耀华，唐春丽，阙祖亮，庾延和，张昕，庞丹清 |
| AUTHORS： | WEI Jiangcun，CHEN Yong，XIE Zhen，LI Yaohua，TANG Chunli，QUE Zuliang，YU Yanhe，ZHANG Xin，PANG Danqing |
| 关键字： | 六棱菊；含量测定；咖啡酸；高效液相色谱法 |
| KEYWORDS： | Laggera alata； Content determination； Caffeic acid； HPLC |
| 阅读数： | 293 次 |
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