猪源纤维蛋白贴对大鼠肝脏及肝素化兔臀大肌创面的止血作用
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篇名: 猪源纤维蛋白贴对大鼠肝脏及肝素化兔臀大肌创面的止血作用
TITLE:
摘要: 目的:研究猪源纤维蛋白贴(DBT)对大鼠肝脏和肝素化兔臀大肌出血创面的止血效果。方法:48只大鼠和24只兔均随机分为假手术组、手术对照组(纱布止血)、医用胶原蛋白海绵组和DBT组,除假手术组外,其他各组动物分别建立大鼠肝脏出血模型或肝素化兔臀大肌静脉出血模型。记录各组动物止血时间、计算出血量;观察大鼠术后3、13周DBT降解、肝脏创面愈合及粘连情况;考察医用胶原蛋白海绵组和DBT组肝素化兔的再渗血率。结果:与假手术组比较,手术对照组动物的止血时间和出血量均显著升高(P<0.01);与手术对照组比较,DBT组和医用胶原蛋白海绵组动物的止血时间和出血量均显著减少(P<0.01)。术后3周和13周,各组大鼠创面均有不同程度粘连,但粘连评分比较差异无统计学意义(P>0.05);术后13周两给药组大鼠肝脏肝缘稍钝,但基本已恢复至术前状态,愈合状况良好;DBT能降解、吸收完全。DBT组兔的再渗血率为33.3%,医用胶原蛋白海绵组为66.7%。结论:DBT对脆性脏器和凝血功能障碍机体具有较好的止血效果,且能降解吸收;其作用效果与医用胶原蛋白海绵相当,且黏合强度略强于后者。
ABSTRACT: OBJECTIVE: To study the hemostatic effect of porcine fibrin sealant patch (DBT) on bleeding wound of liver in rats and gluteus maximus in heparinized rabbits. METHODS: 48 rats and 24 rabbits were randomly divided into sham operation group, operation control group (gauze hemostasis), medical collagen sponge group and DBT group. Except for sham operation group, animals in other groups were reduced for rat model with liver bleeding or heparinized rabbit model with gluteus maximus bleeding. The hemostatic time was recorded, bleeding amount was calculated; DBT degradation and wound adhesion in liver after 3, 13 weeks were observed; re-bleeding rate of heparinized rabbits in medical collagen sponge group and DBT group were investigated. RESULTS: Compared with sham operation group, the hemostatic time and bleeding amount of animals in operation control group were significantly increased (P<0.01). Compared with operation control group, the hemostatic time and bleeding amount of animals in DBT group and medical collagen sponge group were significantly reduced (P<0.01). After 3, 13 weeks, different degree of adhesion appeared in the wound of rats in each group, while the adhesion scores had no statistical significances (P>0.05). After 13 weeks, liver margin of rats in administration groups was slightly blunt, but it basically had been restored to preoperative state, with good healing. DBT can be degraded and absorbed completely. The re-bleeding rate of rabbits in DBT group were 33.3%, 66.7% in medical collagen sponge group. CONCLUSIONS: DBT has good hemostatic effect on fragile organs and the body with blood coagulation dysfunction, and can be degraded and absorbed. Its effect is equivalent to medical collagen, while the adhesive strength is slightly better than the latter.
期刊: 2017年第28卷第28期
作者: 李伟达,邓乐君,朱晋辉,陈灶妹,李健,张俊辉,万华印,李茹冰
AUTHORS: LI Weida,DENG Lejun,ZHU Jinhui,CHEN Zaomei,LI Jian,ZHANG Junhui,WAN Huayin,LI Rubing
关键字: 纤维蛋白贴;肝脏出血;臀大肌出血;止血;凝血功能障碍;大鼠;兔
KEYWORDS: Fibrin sealant patch; Hepatorrhagia; Gluteus maximus bleeding; Hemostasis; Blood coagulation dysfunction; Rats; Rabbits
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