驱虫斑鸠菊中紫铆素对人永生化角质形成细胞株HaCaT增殖及细胞分泌因子的影响
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篇名: 驱虫斑鸠菊中紫铆素对人永生化角质形成细胞株HaCaT增殖及细胞分泌因子的影响
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摘要: 目的:研究驱虫斑鸠菊(VW)中紫铆素对人永生化角质形成细胞株HaCaT的增殖及细胞分泌因子的影响,初步探讨VW中紫铆素治疗白癜风的机制。方法:采用MTT法测定0(空白对照)、0.1、0.5、1.0、5.0、10.0 μg/mL紫铆素作用于HaCaT细胞48 h后的细胞存活率;采用酶联免疫吸附法测定0.5、1.0、5.0 μg/mL紫铆素作用于HaCaT细胞48 h后培养液中细胞分泌因子内皮素1(ET-1)、ET-3、黑素细胞刺激素(MSH)、干细胞因子(SCF)、碱性纤维细胞生长因子(bFGF)的含量。结果:与空白对照比较,0.1~5.0 μg/mL紫铆素作用48 h后细胞存活率均有不同程度升高,而10.0 μg/mL紫铆素作用后细胞存活率降低;0.5、1.0、5.0 μg/mL紫铆素作用48 h后培养液中ET-1、SCF、bFGF含量均显著增加(P<0.01),1.0、5.0 μg/mL紫铆素作用48 h后培养液中ET-3、MSH含量显著增加(P<0.01)。结论:紫铆素可促进HaCaT细胞的增殖,其作用机制可能与促进细胞分泌因子ET-1、ET-3、MSH、SCF、bFGF的分泌有关。
ABSTRACT: OBJECTIVE: To study the effects of butin in Vernohia anthelmintica (VW) on proliferation of human immortal keratinocyte cell strain HaCaT and cell secretory factors, and explore the mechanism of butin in VW in the treatment of vitiligo. METHODS: MTT method was used to determine the survival rate of HaCaT cells cultured by 0 (blank control), 0.1, 0.5, 1.0, 5.0, 10.0 μg/mL of butin for 48 h. Enzyme-linked immunosorbent assay was used to determine the contents of cell secretory factors as endothelin 1 (ET-1), ET-3, melanocyte stimulating hormone (MSH), stem cell factor (SCF), basic fibroblast growth factor (bFGF) in culture medium after HaCaT cells were cultured by 0.5, 1.0, 5.0 μg/mL of butin for 48 h. RESULTS: Compared with blank control, cell survival rate was increased to varying degrees after cultured by 0.1-5.0 μg/mL of butin for 48 h, while decreased after cultured by 10.0 μg/mL of butin. Contents of ET-1, SCF, bFGF in culture medium were significantly increased after cultured by 0.5, 1.0, 5.0 μg/mL of butin for 48 h (P<0.01); and contents of ET-3, MSH in culture medium were significantly increased after cultured by 1.0, 5.0 μg/mL of butin for 48 h (P<0.01). CONCLUSIONS: Butin can promote the proliferation of HaCaT cells, the mechanism may be associated with promoting the secretion of cell secretory factors of ET-1, ET-3, MSH, SCF, bFGF.
期刊: 2017年第28卷第28期
作者: 王志杰,高莉,霍仕霞,谭雪,罗静莺,闫明
AUTHORS: WANG Zhijie,GAO Li,HUO Shixia,TAN Xue,LUO Jingying,YAN Ming
关键字: 驱虫斑鸠菊;紫铆素;人永生化角质形成细胞株HaCaT细胞;增殖;细胞因子
KEYWORDS: Vernohia anthelmintica; Butin; Human immortal keratinocyte cell strain HaCaT; Proliferation; Cell factors
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