RP-HPLC法同时测定加味补阳还五汤剂中4种成分的含量
x

请在关注微信后,向客服人员索取文件

篇名: RP-HPLC法同时测定加味补阳还五汤剂中4种成分的含量
TITLE:
摘要: 目的:建立同时测定加味补阳还五汤剂中芍药苷、苦杏仁苷、阿魏酸、川芎嗪含量的方法。方法:采用反相高效液相色谱法。色谱柱为YMC C18,流动相为乙腈-0.1%磷酸溶液(梯度洗脱),流速为1.0 mL/min,检测波长为320 nm(阿魏酸)、230 nm(芍药苷)、207 nm(苦杏仁苷)、280 nm(川芎嗪),柱温为30 ℃,进样量为10 μL。结果:芍药苷、苦杏仁苷、阿魏酸和川芎嗪检测质量浓度线性范围分别为0.191 2~1.912 μg/mL(r=0.999 6)、0.117 4~1.174 μg/mL(r=0.999 6)、0.011 5~0.115 μg/mL(r=0.999 8)和0.001 66~0.016 6 μg/mL(r=0.999 7);定量限分别为1.912、1.174、0.115、0.016 6 μg/mL ,检测限分别为0.25、0.40、0.05、0.008 5 μg/mL;精密度、稳定性、重复性试验的RSD<2.0%;加样回收率分别为96.9%~100.3%(RSD=1.3%,n=6)、95.1%~100.3%(RSD=2.2%,n=6)、95.3%~100.2%(RSD=2.0%,n=6)、97.0%~100.0%(RSD=1.3%,n=6)。结论:该方法可靠、简便、准确,适用于同时测定加味补阳还五汤剂中芍药苷、苦杏仁苷、阿魏酸、川芎嗪的含量。
ABSTRACT: OBJECTIVE: To establish the method for simultaneous determination of paeoniflorin, amygdalin, ferulic acid and ligustrazine in Modified buying huanwu decoction. METHODS: RP-HPLC method was adopted. The determination was performed on YMC C18 column with the mobile phase consisted of acetonitrile-0.1% phosphoric acid (gradient elution) at the flow rate of 1.0 mL/min. The detection wavelengths were set at 320 nm (ferulic acid), 230 nm (paeoniflorin), 207 nm (amygdalin), 280 nm (ligustrazine). The column temperature was 30 ℃, and sample size was 10 μL. RESULTS: The linear ranges of paeoniflorin, amygdalin, ferulic acid and ligustrazine were 0.191 2-1.912 μg/mL (r=0.999 6),0.117 4-1.174 μg/mL (r=0.999 6),0.011 5-0.115 μg/mL (r=0.999 8) and 0.001 66-0.016 6 μg/mL(r=0.999 7), respectively. The limits of quantitation were 1.912, 1.174, 0.115, 0.016 6 μg/mL, and the limits of detection were 0.25, 0.40, 0.05, 0.008 5 μg/mL, respectively. RSDs of precision, stability and reproducible tests were all lower than 2.0%. The recoveries were 96.9%-100.3%(RSD=1.3%,n=6),95.1%-100.3%(RSD=2.2%,n=6),95.3%-100.2%(RSD=2.0%,n=6)and 97.0%-100.0%(RSD=1.3%, n=6). CONCLUSIONS: The method is reliable, simple and accurate, and is suitable for simultaneous determination of paeoniflorin, amygdalin, ferulic acid and ligustrazine in Modified buyang huanwu decoction.
期刊: 2017年第28卷第27期
作者: 王忠成,顾尔莉,王身艳,王芳芳,俞冲,朱敏
AUTHORS: WANG Zhongcheng,GU Erli,WANG Shenyan,WANG Fangfang,YU Chong,ZHU Min
关键字: 反相高效液相色谱法;加味补阳还五汤;芍药苷;苦杏仁苷;阿魏酸;川芎嗪;含量测定
KEYWORDS: RP-HPLC; Modified buyang huanwu decoction; Paeoniflorin; Amygdalin; Ferulic acid; Ligustrazine; Content determination
阅读数: 198 次
本月下载数: 5 次

* 注:未经本站明确许可,任何网站不得非法盗链资源下载连接及抄袭本站原创内容资源!在此感谢您的支持与合作!