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RGD肽修饰的异长春花碱-粉防己碱脂质体的制备及体外抑瘤作用研究
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| 篇名： | RGD肽修饰的异长春花碱-粉防己碱脂质体的制备及体外抑瘤作用研究 |
| TITLE： | |
| 摘要： | 目的：制备RGD肽修饰的异长春花碱（VRB）-粉防己碱（TET）脂质体，研究其对脑胶质瘤C6细胞的抑制作用。方法：采用薄膜分散法和硫酸铵梯度法制备RGD肽修饰的VRB-TET脂质体，观察其形态和粒径分布，测定其中VRB的含量；以磺酰罗丹明B法分别测定空白靶向脂质体、VRB普通脂质体和RGD肽修饰的VRB-TET脂质体对C6细胞的抑制作用。结果：所制RGD肽修饰的VRB-TET脂质体呈圆球状或类圆球状，表面光滑，粒径为120 nm左右，其中VRB的平均含量为28.27 μg/mL（RSD＝0.38％，n＝3）。空白靶向脂质体对C6细胞生长无显著影响；RGD肽修饰的VRB-TET脂质体能明显抑制C6细胞生长，其作用后细胞存活率明显低于VRB普通脂质体（P＜0.05）。结论：成功制得RGD肽修饰的VRB-TET脂质体，其具有明显的抑制C6细胞生长的作用。 |
| ABSTRACT： | OBJECTIVE： To prepare the vinorelbine-tetrandrine liposomes modified with RGD， and study the inhibitory effect on glioma C6 cells. METHODS： Film dispersion method and ammonium sulfate gradient method were used to prepare the vinorelbine-tetrandrine liposomes modified with RGD， and the morphology and particle size distribution were observed. The vinorelbine content was determined， and sulforhodamine B method was used to respectively determine the inhibitory effects of blank targeting liposomes， normal vinorelbine liposomes and vinorelbine-tetrandrine liposomes modified with RGD on C6 cells. RESULTS： The prepared vinorelbine-tetrandrine liposomes modified with RGD were spherical or almost spherical with smooth surface， and particle size was about 120 nm. The average content of vinorelbine was 28.27 μg/mL （RSD＝0.38％， n＝3）. Blank targeting liposomes had no significant effect on the growth of C6 cells； vinorelbine-tetrandrine liposomes modified with RGD can obviously inhibit the growth of C6 cells， and cell viability after its effect was significantly lower than normal vinorelbine liposomes （P＜0.05）. CONCLUSIONS： Vinorelbine-tetrandrine liposomes modified with RGD are successfully prepared， and they show obvious inhibitory effects on the growth of C6 cells. |
| 期刊： | 2017年第28卷第25期 |
| 作者： | 居瑞军，王晓敏，晁建平，程岚，李学涛 |
| AUTHORS： | JU Ruijun，WANG Xiaomin，CHAO Jianping，CHENG Lan，LI Xuetao |
| 关键字： | RGD肽；异长春花碱；粉防己碱；脂质体；体外抑瘤作用 |
| KEYWORDS： | RGD； Vinorelbine； Tetrandrine； Liposomes； in vitro antitumor effect |
| 阅读数： | 299 次 |
| 本月下载数： | 3 次 |
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