泽泻、郁金、熟地黄合煎对熟地黄中毛蕊花糖苷煎出量的影响
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篇名: 泽泻、郁金、熟地黄合煎对熟地黄中毛蕊花糖苷煎出量的影响
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摘要: 目的:研究泽泻、郁金、熟地黄合煎对熟地黄中毛蕊花糖苷煎出量的影响,为三药合用的药效物质研究提供参考。方法:取药材分为单煎-1(熟地黄 20 g)、单煎-2(泽泻20 g)、单煎-3(郁金20 g)、合煎-1(熟地黄、泽泻各20 g)、合煎-2(熟地黄、郁金各20 g)、合煎-3(泽泻、郁金各20 g)、合煎-4(熟地黄、泽泻、郁金各20 g)组,分别制备水煎液干浸膏,回流法提取后采用高效液相色谱法检测样品液中毛蕊花糖苷的含量并计算其煎出量。结果:单煎-1、合煎-1、合煎-2、合煎-4干浸膏中毛蕊花糖苷煎出量分别约为0.035 4、0.022 3、0.022 8、0.011 0 mg/g,后3组与单煎-1组比较差异具有统计学意义(P<0.01),以三药合煎组煎出量最低;阴性对照组(单煎-2、单煎-3及合煎-3)中未检测到毛蕊花糖苷。结论:熟地黄与泽泻、郁金三药合煎时毛蕊花糖苷煎出量降低,推测毛蕊花糖苷可能不是三药合煎液中发挥药效作用的主要成分。
ABSTRACT: OBJECTIVE: To study the effects of combined decoctions of alismatis rhizoma and curcumae radix and rehmanniae radix praeparata on the decoction amount of acteoside in rehmanniae radix praeparata, and provide reference for studying the effective ingredients of three-drug effect. METHODS: Single decoction-1 (rehmanniae radix praeparata 20 g), single decoction-2 (alismatis rhizoma 20 g), single decoction-3 (curcumae radix 20 g), combined decoction-1 (rehmanniae radix praeparata 20 g, alismatis rhizoma 20 g), combined decoction-2 (rehmanniae radix praeparata 20 g, curcumae radix 20 g), combined decoction-3 (alismatis rhizoma 20 g, curcumae radix 20 g) and combined decoction-4 (rehmanniae radix praeparata 20 g, alismatis rhizoma 20 g, curcumae radix 20 g) were respectively taken to prepare dry extracts after extracting by refluxing, HPLC was used to detect the acteoside content and calculate its decoction amount in sample. RESULTS: The decoction amounts of acteoside in single decoction-1, combined decoction-1, combined decoction-2 and combined decoction-4 dry extracts were 0.035 4, 0.022 3, 0.022 8, 0.011 0 mg/g, respectively. Compared with single decoction-1 group, the last 3 groups had statistical significances (P<0.01); combined decoction-4 showed lowest decoction amount in three-drug combined decoction group. Acteoside was not detected in the negative control groups (single decoction-2, single decoction-3 and combined decoction-3). CONCLUSIONS: The decoction amount of acteoside is reduced when alismatis rhizoma, curcumae radix and rehmanniae radix praeparata were decocted t
期刊: 2017年第28卷第25期
作者: 袁晓旭,杨明明,王慧娜,李洪波,缪红,赵桂琴
AUTHORS: YUAN Xiaoxu,YANG Mingming,WANG Huina,LI Hongbo,MIAO Hong,ZHAO Guiqin
关键字: 熟地黄;泽泻;郁金;毛蕊花糖苷;高效液相色谱法;煎出量
KEYWORDS: Rehmanniae radix praeparata; Alismatis rhizoma; Curcumae radix; Acteoside; HPLC; Decoction amount
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