山银花水提物体外抗甲型H1N1流感病毒的作用研究
x

请在关注微信后,向客服人员索取文件

篇名: 山银花水提物体外抗甲型H1N1流感病毒的作用研究
TITLE:
摘要: 目的:考察山银花水提物(SYHW)体外抗甲型H1N1流感病毒(H1N1病毒)的作用。方法:以H1N1病毒感染体外培养的犬肾小管上皮细胞(MDCK细胞),计算病毒的半数组织培养感染剂量(TCID50);以不同质量浓度SYHW作用MDCK细胞24 h,考察其最大无毒浓度。然后将试验分为正常细胞组、病毒对照组和SYHW预防给药组、治疗给药组和直接杀灭给药组(均给予最大无毒浓度的SYHW,并以100 TCID50 H1N1病毒感染细胞),测定SYHW的抗病毒有效率(ER);将试验分为正常细胞组、病毒对照组和SYHW治疗给药组、直接杀灭给药组(给药及病毒感染方法同上),分别测定细胞增殖指数(PI)和细胞凋亡率的变化。结果:H1N1病毒的100 TCID50为1.26×10-7,SYHW对MDCK细胞的最大无毒浓度为50 μg/mL(细胞存活率为91.3%)。SYHW预防给药组、SYHW治疗给药组和SYHW直接杀灭给药组的ER分别为0、80.3%、52.7%。与正常细胞组比较,病毒对照组细胞的PI值显著降低(P<0.05),早期、晚期凋亡率显著升高(P<0.05);与病毒对照组比较,SYHW直接杀灭给药组细胞的PI值显著升高、早期凋亡率显著降低(P<0.05),SYHW治疗给药组细胞的早期凋亡率显著降低(P<0.05)。结论:SYHW具有体外抗H1N1病毒的作用,且以治疗给药和直接杀灭给药抗病毒作用较好。
ABSTRACT: OBJECTIVE: To investigate the effect of aqueous extraction of Lonicerae Flos (SYHW) on anti-influenza A virus H1N1 (H1N1 virus) in vitro. METHODS: Using Madin-Darby canine kid ney (MDCK) cells cultured in vitro by H1N1 virus, half of the tissue culture infection dose (TCID50) was calculated. Culturing MDCK cells for 24 h with different mass concentrations of SYHW, the maximum non-toxic concentration was investigated. And then test was divided into normal cell group, virus control group, SYHW preventive administration group, therapeutic administration group and direct killing group (given SYHW of maximum non-toxic concentration, infecting cells by 100 TCID50 H1N1 virus), and antiviral effective rate (ER) of SYHW was determined. Test was divided into normal cell group, virus control group, SYHW therapeutic group and direct killing group (the same administration and infection as above), changes of cell proliferation index (PI) and cell apoptosis rate were respectively determined. RESULTS: 100 TCID50 of H1N1 virus was 1.26×10-7, and the maximum non-toxic concentration of SYHW on MDCK cells was 50 μg/mL (cell survival rate was 91.3%). ERs of preventive administration group, therapeutic administration group and direct killing group were 0, 80.3% and 52.7%, respectively. Compared with normal cell group, PI value in virus control group was significantly reduced (P<0.05), early and late apoptotic rates were significantly increased (P<0.05). Compared with virus control group, PI value in directly killing group was significantly increased (P<0.05), and early apoptotic rate was significantly reduced (P<0.05); early apoptotic rates in therapeutic administration group were significantly reduced (P<0.05). CONCLUSIONS: SYHW shows anti-H1N1 virus effect in vitro, therapeutic administration and directly killing are preferred in antiviral effect.
期刊: 2017年第28卷第16期
作者: 陈灵,周艳萌,欧水平,任丽
AUTHORS: CHEN Ling,ZHOU Yanmeng,OU Shuiping,REN Li
关键字: 山银花;水提物;MDCK细胞;甲型H1N1流感病毒;体外
KEYWORDS: Lonicerae Flos; Aqueous extraction; MDCK cell; Influenza A virus H1N1; in vitro
总下载数: 2 次
本日下载数: 0次
本月下载数: 0次
文件大小: 619.60Kb

* 注:未经本站明确许可,任何网站不得非法盗链资源下载连接及抄袭本站原创内容资源!在此感谢您的支持与合作!