葛根芩连结肠定位片对湿热内蕴型溃疡性结肠炎模型兔结肠组织PPAR-γ、NF-κB p65蛋白表达的影响
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篇名: 葛根芩连结肠定位片对湿热内蕴型溃疡性结肠炎模型兔结肠组织PPAR-γ、NF-κB p65蛋白表达的影响
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摘要: 目的:观察葛根芩连结肠定位片(GGQLJC)对湿热内蕴型溃疡性结肠炎(UC)模型兔结肠组织过氧化物酶增殖物激活受体(PPAR-γ)和核因子κB(NF-κB p65)蛋白表达的影响。方法:将56只家兔随机分为正常组(生理盐水)、模型组(生理盐水)、柳氮磺胺吡啶片(SASP)组(阳性对照,0.300 g/kg)、葛根芩连片(GGQL)组(0.225 g/kg)和GGQLJC高、中、低剂量组(1.036、0.518、0.259 g/kg),每组8只。除正常组外,其余各组兔复制湿热内蕴型UC模型,于造模结束后次日ig给药,每天1次,连续14 d。对兔疾病活动度指数(DAI)、结肠黏膜损伤指数(CMDI)、组织学损伤(TDI)进行评分,测定结肠、脾、胸腺指数,并检测结肠组织中PPAR-γ、NF-κB p65蛋白表达。结果:与正常组比较,模型组兔DAI 、CMDI和TDI评分以及结肠指数、脾指数、结肠组织中NF-κB p65蛋白表达水平均显著升高(P<0.01),胸腺指数、结肠组织中PPAR-γ蛋白表达水平均显著降低(P<0.01);与模型组比较,各给药组兔上述指标均显著改善(P<0.05或P<0.01);与GGQL组比较,SASP 组和GGQLJC高、中剂量组兔DAI和TDI评分、脾指数、结肠指数、结肠组织中NF-κB p65蛋白表达水平以及SASP 组和GGQLJC高剂量组兔CMDI评分均显著降低(P<0.05),SASP 组和GGQLJC高、中剂量组兔结肠组织中PPAR-γ蛋白表达水平显著升高(P<0.05或P<0.01)。结论:GGQLJC对湿热内蕴型UC模型兔有一定改善作用,且作用优于GGQL;其机制可能与上调结肠组织中PPAR-γ蛋白表达和下调NF-κB p65蛋白表达有关。
ABSTRACT: OBJECTIVE: To observe the effects of Gegenqinlian colon positioning tablet (GGQLJC) on colon tissue PPAR-γ, NF-κB p65 protein expressions of model rabbits with damp-heat type ulcerative colitis (UC). METHODS: 56 rabbits were randomly divided into normal group (normal saline), model group (normal saline), sulfasalazine tablet (SASP) group (positive control, 0.300 g/kg), Gegenqinlian tablet (GGQL) group (0.225 g/kg) and GGQLJC high-dose, medium-dose, low-dose groups (1.036, 0.518, 0.259 g/kg), 8 in each group. Except for normal group, rabbits in other groups were cultured for damp-heat-type UC model, intragastrically administrated in the second day of last administration, once a day, for 14 d. Disease activity index (DAI), colonic mucosal damage index (CMDI), histological damage (TDI) were scored; colon, spleen and thymus indexes were determined; PPAR-γ, NF-κB p65 protein expressions in colon tissue were detected. RESULTS: Compared with normal group, DAI, CMDI, TDI scores and spleen index, colon index, NF-κB p65 protein level in colon tissue in model group were significantly increased (P<0.01); thymus index, PPAR-γ protein level in colon tissue were significantly reduced (P<0.01). Compared with model group, above-mentioned indexes in each administration group were significantly improved (P<0.05 or P<0.01). Compared with GGQL group, DAI and TDI scores, spleen index, colon index, NF-κB p65 protein level in colon tissue in SASP group, GGQLJC high-dose, medium-dose groups were significantly decreased (P<0.05); PPAR-γ protein level in colon tissue in SASP group, GGQLJC high-dose, medium-dose groups were significantly increased (P<0.05 or P<0.01). CONCLUSIONS: GGQLJC has certain improvement effects on model rabbits with damp-heat type UC, which is superior to GGQL. The mechanism may be related to increasing PPAR-γ protein level and decreasing NF-κB p65 protein level in colon tissue.
期刊: 2017年第28卷第16期
作者: 宋信莉,张石宇,郭光伟,刘文,章宏
AUTHORS: SONG Xinli,ZHANG Shiyu,GUO Guangwei,LIU Wen,ZHANG Hong
关键字: 葛根芩连结肠定位片;湿热内蕴型溃疡性结肠炎;过氧化物酶增殖物激活受体;核因子κB;家兔
KEYWORDS: Gegenqinlian colon positioning tablet; Damp-heat type ulcerative colitis; PPAR-γ; NF-κB p65; Rabbit
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