小叶莲有效成分对人乳腺癌细胞增殖、细胞周期及线粒体膜电位的影响
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篇名: 小叶莲有效成分对人乳腺癌细胞增殖、细胞周期及线粒体膜电位的影响
TITLE:
摘要: 目的:探讨小叶莲提取物、有效成分及成分组合对人乳腺癌细胞增殖的影响及机制。方法:采用酸性磷酸酶法测定小叶莲4种提取物[乙醇提取物(Xc)、乙醇提取物石油醚萃取物(Xp)、乙醇提取物乙酸乙酯萃取物(Xe)、乙醇提取物正丁醇萃取物(Xz)]、5种有效成分[鬼臼毒素(S1)、去氧鬼臼毒素(S2)、4-去甲去氧鬼臼毒素(S3)、8-异戊烯基山柰酚(S4)、8,2′ -二异戊烯基槲皮素-3-甲醚(S5)]以及3种有效成分组合{组合1[S1-S2-S3-S4-S5(2 ∶ 4 ∶ 1 ∶ 4 ∶ 32),Z1]、组合2[S2-S4(1 ∶ 1),Z2]、组合3[S3-S4(1 ∶ 4),Z3]}对人乳腺癌MDA-MB-231、MCF-7细胞增殖的影响;流式细胞仪检测上述样品对MDA-MB-231、MCF-7(或T47D)细胞周期和细胞线粒体膜电位的影响。结果:小叶莲有效成分组合Z1对MDA-MB-231、MCF-7细胞具有显著抑制作用,其半数抑制浓度(IC50)分别为(0.27±0.2)、(0.11±0.1) μg/mL;提取物Xc、Xp、Xe,有效成分S2、S4以及成分组合Z2、Z3对MDA-MB-231、MCF-7(或T47D)细胞的增殖均有较强抑制作用,IC50均小于15 μg/mL;各提取物和有效成分均可阻滞MDA-MB-231、MCF-7细胞周期于G2/M期;各有效成分组合均可阻滞MDA-MB-231、T47D细胞周期于G0/G1期,并能降低MDA-MB-231、T47D细胞线粒体膜电位。结论:小叶莲有效成分及成分组合可通过影响细胞周期和线粒体膜电位,从而抑制乳腺癌细胞的增殖。
ABSTRACT: OBJECTIVE: To explore the effects and mechanism of extracts, active constituents and constituent combination of Sinopodophylli Fructus on cell proliferation of human breast cancer. METHODS: Acid phosphatase method was conducted to determine the effects of 4 extracts [ethanol extract (Xc), petroleum ether extract from ethanol extract (Xp), ethyl acetate extract from ethanol extract (Xe), n-butanol extract from ethanol extract (Xz)], 5 active constituents [podophyllotoxin (S1), deoxypodophyllotoxin (S2), 4-desmethyl deoxypodophyllotoxin (S3), 8-isopentenyl kaempferol (S4), 8,2′ -diisoprenyl quercetin-3-methyl ether (S5)] and 3 active constituent combination [combination 1, S1-S2-S3-S4-S5 (2 ∶ 4 ∶ 1 ∶ 4 ∶ 32), Z1; combination 2, S2-S4 (1 ∶ 1), Z2; combination 3, S3-S4 (1 ∶ 4), Z3] on the MDA-MB-231, MCF-7 cell proliferation; flow cytometry was adopted to detect the effects of above-mentioned samples on MDA-MB-231, MCF-7 (T47D) cell cycle and mitochondrial membrane potential. RESULTS: The active constituent combination Z1 showed significant inhibitory effects on MDA-MB-231, MCF-7 cells, the half inhibitory concentrations (IC50) were (0.27±0.2), (0.11±0.1) μg/mL; extracts Xc, Xp, Xe, active constituents S2, S4 and active constituent combination Z2, Z3 showed relatively strong inhibitory effects on MDA-MB-231, MCF-7 (T47D) cell proliferation (IC50<15 μg/mL). Both extracts and active constituents can block MDA-MB-231, MCF-7 cell cycle in G2/M phase; all active constituents can block MDA-MB-231, T47D cell cycle in G0/G1 phase, and can reduce MDA-MB-231, T47D cell mitochondrial membrane potential. CONCLUSIONS: The active constituents and constituent combination of Sinopodophylli Fructus can inhibit cell proliferation of breast cancer by affecting cell cycle and mitochondrial membrane potential.
期刊: 2017年第28卷第10期
作者: 孔越,王庆辉,尚明英,肖军军,孟书聪,蔡少青
AUTHORS: KONG Yue,WANG Qinghui,SHANG Mingying,XIAO Junjun,MENG Shucong,CAI Shaoqing
关键字: 小叶莲;有效成分;有效成分组合;人乳腺癌MDA-MB-231细胞;人乳腺癌MCF-7细胞;人乳腺癌T47D细胞;细胞周期;线粒体膜电位
KEYWORDS: Sinopodophylli Fructus; Active constituent; Active constituent combination; Human breast cancer MDA- MB-231 cells; Human breast cancer MCF-7 cells; Human breast cancer T47D cells; Cell cycle;Mitochondrial membrane potential
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