东北地区铁线透骨草药材的HPLC指纹图谱研究
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篇名: 东北地区铁线透骨草药材的HPLC指纹图谱研究
TITLE:
摘要: 目的: 建立铁线透骨草药材的高效液相色谱(HPLC)指纹图谱。方法:采用HPLC法。色谱柱为Hedera ODS-2 C18,流动相为乙腈-0.5%磷酸溶液(梯度洗脱),流速为1.0 mL/min,检测波长为338 nm,柱温为30 ℃,进样量为20 μL。以芦丁为参照物,测定10批铁线透骨草药材的HPLC图谱,采用《中药色谱指纹图谱相似度评价系统》(2004A版)进行共有峰确定和相似度评价。结果: 10批铁线透骨草药材有16个共有峰,相似度均>0.9。经验证,10批药材样品HPLC图谱与对照指纹图谱具有较好的一致性。结论:所建指纹图谱可为东北地区铁线透骨草药材的鉴别和质量评价提供参考。
ABSTRACT: OBJECTIVE: To establish the HPLC fingerprints for Herba clematidis in northeast. METHODS: HPLC was performed on the column of Hedera ODS-2 C18 with mobile phase of acetonitrile-0.5% phosphoric acid solution (gradient elution) at a flow rate of 1.0 mL/min, detection wavelength was 338 nm, column temperature was 30 ℃, and the injection volume was 20 μL. Using rutin as as a reference,the HPLC profiles of 10 batches of H. clematidis were determined, Similarity Evaluation Software for Chromatographic Fingerprint of Traditional Chinese Medicine(2004A edition) was used for the common peaks identification and similarity evaluation. RESULTS: There were 16 common peaks in the 10 batches of H. clematidis, similarity degree was higher than 0.9. It was proved that the HPLC profiles and control fingerprint profile of 10 batches of H. clematidis had good consistency. CONCLUSIONS: The established fingerprints can provide reference for the identification and quality evaluation of H. clematidis in northeast.
期刊: 2017年第28卷第9期
作者: 贾媛,张恒玉,柴尧,彭九嫚,杜俊潮
AUTHORS: JIA Yuan,ZHANG Hengyu,CHAI Yao,PENG Jiuman,DU Junchao
关键字: 东北地区;铁线透骨草;高效液相色谱法;指纹图谱
KEYWORDS: Northeast; Herba clematidis; HPLC; Fingerprint chromatogram
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