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UPLC-PDA法测定藏药翼首草不同药用部位中齐墩果酸和熊果酸的含量
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| 篇名： | UPLC-PDA法测定藏药翼首草不同药用部位中齐墩果酸和熊果酸的含量 |
| TITLE： | |
| 摘要： | 目的：建立测定藏药翼首草不同药用部位中齐墩果酸和熊果酸含量的方法，并比较其不同部位之间的含量差异。方法：采用超高效液相色谱-光电二极管阵列检测器（UPLC-PDA）测定翼首草不同药用部位（全草、地上部位和地下部位）齐墩果酸和熊果酸含量。色谱柱为Acquity UPLC HSS T3（150 mm×2.1 mm，1.8 μm）；流动相为甲醇-0.1 mol/L乙酸铵溶液（88 ∶ 12，V/V），流速为0.2 mL/min；检测波长为210 nm；柱温为30 ℃；进样量为5 μL。结果：齐墩果酸和熊果酸的检测质量浓度线性范围分别为10.65～1 065 μg/mL（r＝0.999 6）、18.8～1 880 μg/mL（r＝0.999 4），平均加样回收率分别为96.95％（RSD＝1.24％，n＝9）、98.12％（RSD＝2.13％，n＝9），精密度、稳定性、重复性试验的RSD均小于3％。不同药用部位的齐墩果酸和熊果酸含量的高低整体趋势为地上部位＞全草＞地下部位；翼首草全草中齐墩果酸和熊果酸平均总量为0.35％，地上部位中为0.56％，地下部位中为0.09％。结论：建立的方法快速、准确可靠、重复性好，适合于藏药翼首草不同药用部位中齐墩果酸和熊果酸含量的测定。翼首草地上部位中齐墩果酸和熊果酸含量较全草、地下部位高，建议使用地上部位入药。 |
| ABSTRACT： | OBJECTIVE： To establish the method for the determination of oleanolic acid and ursolic acid in different medicinal parts of Tibetan medicine Pteocephalus hookeri， and compare the differences among the different parts. METHODS： The contents of oleanolic acid and ursolic acid from different medicinal parts （whole plant， aerial part， underground part） of P. hookeri were determined by UPLC-PDA. The separation was performed on Acquity UPLC HSS T3 column（150 mm×2.1 mm，1.8 μm） with mobile phase consisted of methanol-0.1 mol/L ammonium acetate （88 ∶ 12， V/V） at the flow rate of 0.2 mL/min. The detection wavelength was set at 210 nm， and column temperature was 30 ℃. The sample size was 5 μL. RESULTS： The linear ranges of oleanolic acid and ursolic acid were 10.65-1 065 μg/mL （r＝0.999 6） and 18.8-1 880 μg/mL （r＝0.999 4）， separately. The recoveries were 96.95％ （RSD＝1.24％， n＝9） and 98.12％ （RSD＝2.13％， n＝9）， separately. RSDs of precision， stability and reproducibility tests were all less than 3％. The contents of oleanolic acid and ursolic acid from different medicinal parts in P. hookeri were in descending order of aerial part＞whole plant＞underground part； the average total content of oleanolic acid and ursolic acid from whole plants was 0.35％， the aerial part reached 0.56％ and underground part was 0.09％. CONCLUSIONS： The method is rapid， accurate and reproducible， and it is suitable for the content determination of oleanolic acid and ursolic acid in different medicinal parts of Tibetan medicine P. hookeri. The contents of oleanolic acid and ursolic acid from aerial part of P. hookeri are higher than whole plant and underground part. It is suggested to use aerial parts of medicine. |
| 期刊： | 2017年第28卷第7期 |
| 作者： | 唐策，苏锦松，杨娟，左芳，孟宪丽，邹忠梅，张艺 |
| AUTHORS： | TANG Ce，SU Jinsong，YANG Juan，ZUO Fang，MENG Xianli，ZOU Zhongmei，ZHANG Yi |
| 关键字： | 藏药；翼首草；超高效液相色谱；光电二极管阵列检测器；药用部位；齐墩果酸；熊果酸；含量测定 |
| KEYWORDS： | Tibetan medicine； Pteocephalus hookeri； UPLC； PDA； Medicinal parts； Oleanolic acid； Ursolic acid； Content determination |
| 阅读数： | 288 次 |
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