藏药复方多血康胶囊对脂多糖诱导急性肺损伤小鼠的保护作用
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篇名: 藏药复方多血康胶囊对脂多糖诱导急性肺损伤小鼠的保护作用
TITLE:
摘要: 目的:考察藏药复方多血康胶囊对脂多糖诱导急性肺损伤小鼠的保护作用。方法:将60只KM小鼠随机分为空白组、模型组、地塞米松组(阳性对照,1 mg/kg)和复方多血康胶囊高、中、低剂量组(3.6、1.8、0.9 g/kg,以生药计),每组10只。ig给药,空白组和模型组小鼠ig等体积生理盐水,每天1次。连续给药7 d后,除空白组外,其余各组小鼠均ip脂多糖诱导急性肺损伤。造模6 h后,观察小鼠肺组织病理改变,测量肺组织含水量,检测肺组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)水平以及血清中白细胞介素6(IL-6)、肿瘤坏死因子α(TNF-α)水平。结果:与空白组比较,模型组小鼠肺组织发生充血水肿、炎症细胞浸润、肺泡间隔及肺泡壁明显增宽等病理变化,肺组织含水量和肺组织中MDA水平以及血清中IL-6、TNF-α水平均显著升高(P<0.01),肺组织中SOD、GSH-Px水平显著降低(P<0.05)。与模型组比较,各给药组小鼠肺组织损伤程度均不同程度降低;除复方多血康胶囊低剂量组大鼠肺组织含水量、肺组织中MDA水平以及血清中TNF-α水平变化不明显外,其余各给药组小鼠上述指标均显著改善(P<0.05或P<0.01)。结论:藏药复方多血康胶囊可明显减轻小鼠氧化应激和炎症反应,对脂多糖诱导的急性肺损伤小鼠有一定保护作用。
ABSTRACT: OBJECTIVE: To investigate the protective effect of Tibetan medicine Compound Duoxuekang capsules on the acute lung injury in mice induced by lipopolysaccharide. METHODS: 60 KM mice were randomly divided into blank group, model group, dexamethasone group (positive control, 1 mg/kg) and Compound Duoxuekang high-dose, medium-dose, low-dose groups (3.6, 1.8, 0.9 g/kg, calculated by crude drug),10 in each group. Intragastrically administrated, mice in blank group and model group intragastrically administrated equal volume of normal saline, once a day. After 7 d of administration, except for blank group, mice in other groups intraperitoneally injected lipopolysaccharide to induce acute lung injury. After 6 h modeling, pathological changes in the lung tissue was observed, lung tissue water content was measured, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), malondialdehyde (MDA) levels in lung tissue and IL-6, TNF-α levels in serum were detected. RESULTS: Compared with blank group, mice in model group showed pathological changes in congestion and edema, inflammatory cell infiltration, obvious widened alveolar septum and alveolar wall; water content in lung tissue and IL-6, TNF-α levels in serum were significantly increased (P<0.01); SOD, GSH-Px levels in lung tissue were significantly decreased (P<0.01). Compared with model group, injury degree of lung tissue reduced to varying degrees in each treatment group, except for the water content, MDA level in lung tissue and TNF-α level in serum in Compound Duoxuekang capsules low-dose group; the above-mentioned indexes in other groups were significantly improved (P<0.05 or P<0.01). CONCLUSIONS: Compound Duoxuekang capsules can obviously reduce mice’s oxidative stress and inflammatory response, and has certain protective effect on acute lung injury in mice.
期刊: 2017年第28卷第7期
作者: 胡晓鑫,罗强,杜蕾蕾,张艺,降拥四郎
AUTHORS: HU Xiaoxin,LUO Qiang,DU Leilei,ZHANG Yi,JIANGYONG Silang
关键字: 藏药;复方多血康胶囊;急性肺损伤;炎症;氧化应激;小鼠
KEYWORDS: Tibetan medicine; Compound Duoxuekang capsules ; Acute lung injury; Inflammation; Oxidative stress; Mice
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