银杏叶聚戊烯醇对Aβ25-35诱导dPC12细胞损伤的保护作用研究
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篇名: 银杏叶聚戊烯醇对Aβ25-35诱导dPC12细胞损伤的保护作用研究
TITLE:
摘要: 目的:研究银杏叶聚戊烯醇(GP)对β淀粉样肽25-35(Aβ25-35)诱导dPC12细胞损伤的保护作用,为其用于阿尔茨海默病(AD)的治疗提供参考。方法:以神经生长因子(NGF)诱导PC12细胞分化为具有神经活性的dPC12细胞后,将dPC12细胞分为正常对照组(DMSO培养基)、Aβ25-35处理组(DMSO培养基)和GP试验组(分别含25、50、100、200、400 μg/mL GP的DMSO培养基),培养24 h后,Aβ25-35处理组和GP试验组细胞均加入25 μmol/L Aβ25-35诱导细胞损伤(即复制AD细胞模型),24 h后采用MTT法测定细胞存活率。另取细胞分为正常对照组(DMSO培养基)、Aβ25-35处理组(DMSO培养基)和GP试验组(分别含25、50、100、200     μg/mL GP的DMSO培养基),同法处理后检测细胞培养液中乳酸脱氢酶(LDH)、活性氧(ROS)、丙二醛(MDA)水平。结果:与     Aβ25-35处理组比较,50、100、200、400 μg/mL GP试验组dPC12细胞的存活率明显升高(P<0.05或P<0.01),100、200 μg/mL GP试验组细胞培养液中LDH、ROS和MDA水平以及50 μg/mL GP试验组细胞培养液中ROS水平均显著降低(P<0.05或P<0.01),且呈一定的浓度依赖性。结论:GP对Aβ25-35致dPC12损伤有一定的保护作用,为一种潜在的AD治疗药物。
ABSTRACT: OBJECTIVE: Study the protective effect of the polypentenol from Ginkgo biloba leaf (GP)on Aβ25-35-induced damage in dPC12 cells ,and to provide reference for the treatment of Alzheimer’s disease (AD). METHODS:After PC12 cells were differentiated into dPC12 cells with neural activity by incubating with never growth factor (NGF), the dPC12 cells were divided into normal control group (DMSO medium),Aβ25-35 treatment group (DMSO medium) and GP test group (DMSO medium respectively containing 25,50,100,200,400 μg/mL GP). After 24 h cultivating, cells in Aβ25-35 treatment group and GP test group were added 25 μmol/L Aβ25-35 to induce cell damage (AD cell model), MTT method was used to determine the survival rate after 24 h. Other cells were divided into normal control group (DMSO medium), Aβ25-35 treatment group(DMSO medium)and GP test group(DMSO medium respectively containing 25,50,100,200 μg/mL GP), the lactate dehydrogenase (LDH), reactive oxygen species (ROS), malondialdehyde (MDA) levels in cell culture medium were detected after the same treatment. RESULTS:Compared with Aβ25-35 treatment group, the survival rates of dPC12 cells in 50, 100, 200, 400 μg/mL GP test groups were obviously increased (P<0.05 or P<0.01); LDH, ROS, MDA levels in 100, 200 μg/mL GP test groups and ROS level in 50 μg/mL GP test group were significantly reduced (P<0.05 or P<0.01),showing a certain concentration-dependence. CONCLUSIONS: GP has certain protective effect on Aβ25-35-induced damage in dPC12 cells, and it’s a potential drug for AD.
期刊: 2017年第28卷第7期
作者: 陈卫卫,梁迪,赵其秀,罗毅,刘灵杰,莫丽萍,黄俊善,李海涛
AUTHORS: CHEN Weiwei,LIANG Di,ZHAO Qixiu,LUO Yi,LIU Lingjie,MO Liping,HUANG Junshan,LI Haitao
关键字: 银杏叶;聚戊烯醇;β淀粉样肽25-35;dPC12细胞;乳酸脱氢酶;活性氧;丙二醛
KEYWORDS: Ginkgo biloba leaf; Polypentenol; Aβ25-35; dPC12 cell; Lactate dehydrogenase; Reactive oxygen species; Malondialdehyde
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