基于DNA条形码ITS2序列对阿魏属药用植物的鉴别研究
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篇名: 基于DNA条形码ITS2序列对阿魏属药用植物的鉴别研究
TITLE:
摘要: 目的: 建立一种快速、准确和标准化的中药材阿魏属植物DNA条形码鉴别方法。方法:提取新疆阿魏(Ferula sinkiangensis K. M. Shen)和阜康阿魏(Ferula fukanensis K. M. Shen)基因组DNA,扩增ITS2序列并测序;运用分析相似性搜索法,下载GenBank数据库中阿魏属植物13个物种26个样本基因 ITS2序列并进行比对分析,计算种间种内遗传距离并构建系统进化树进行聚类分析。结果:通过计算,15个物种遗传距离分布范围为0.009~0.230,平均遗传距离为0.018;聚类分析结果显示DNA条形码ITS2序列能够将阿魏属32种植物聚为不同的类。结论:建立的阿魏药材DNA条形码ITS2序列鉴别方法可准确、快速地鉴别出阿魏属药材的正品和混淆品。
ABSTRACT: OBJECTIVE: To establish a rapid, accurate and standardized DNA barcode identification method for the ferulic medicinal plants. METHODS: Genomic DNA was extracted from Ferula sinkiangensis K. M. Shen and Ferula fukanensis K. M. Shen, ITS2 sequences were amplified and sequenced, analytical similarity search method was conducted, 13 species 26 samples gene ITS2 sequences of ferulic medicinal plants in GenBank database were downloaded and comparatively analyzed; interspecies and intraspecies genetic distances were calculated, and phylogenetic tree was developed for cluster analysis. RESULTS: According to calculation, species genetic distances of the 15 species ranged 0.009-0.230, average genetic distance was 0.018; results of cluster analysis showed DNA barcode ITS2 sequences can cluster the 32 kinds of ferulic medicinal plants into different classes. CONCLUSIONS: The established DNA barcode ITS2 sequences identification method can accurately and rapidly identify the genuine and false samples of ferulic species.
期刊: 2017年第28卷第7期
作者: 刘冲,杨伟俊,何江,程波,地力努尔·吐尔逊江
AUTHORS: LIU Chong,YANG Weijun,HE Jiang,CHENG Bo,DILINUER·Tuerxunjiang
关键字: 阿魏属;DNA条形码;ITS2序列;药材;鉴别
KEYWORDS: Ferulic species; DNA barcode; ITS2 sequences; Medicinal materials; Identification
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