杜仲壮骨丸对维甲酸致小鼠骨质疏松的改善作用
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篇名: 杜仲壮骨丸对维甲酸致小鼠骨质疏松的改善作用
TITLE:
摘要: 目的:考察杜仲壮骨丸(DZ)对小鼠骨质疏松的改善作用。方法:将72只KM小鼠随机分为空白对照组(生理盐水)、模型组(生理盐水)、阳性对照组(仙灵骨葆胶囊,0.50 g/kg)和DZ高、中、低剂量组(1.90、0.95、0.48 g/kg,记为DZ-H、DZ-M、DZ-L组),每组12只。除空白对照组外,其余各组小鼠均ig维甲酸复制骨质疏松模型,并同时ig相应药物,每天1次,连续14 d。末次给药后,测定小鼠体质量;摘眼球取血测定血清钙、血清磷含量;取肝、脾、胸腺测定脏器指数;取左侧股骨测定骨湿质量、干质量、长度和直径;取右侧股骨测定骨小梁面积百分比、骨小梁宽度及数目。结果:与空白对照组比较,模型组小鼠体质量降低;血清钙、血清磷含量增加;肝指数、脾指数升高,胸腺指数降低;股骨湿、干质量及直径减少;骨小梁面积百分比降低,骨小梁宽度及数目减少,差异均有统计学意义(P<0.05或P<0.01)。与模型组比较,各给药组小鼠血清钙含量减少,阳性对照组和DZ-M、DZ-L组小鼠血清磷含量减少;阳性对照组和DZ-M、DZ-L组小鼠胸腺指数升高,阳性对照组小鼠肝脏指数降低;阳性对照组小鼠的股骨湿质量及DZ-M组小鼠股骨干、湿质量和直径均增加;阳性对照组和DZ-H、DZ-M组小鼠股骨骨小梁百分比升高,骨小梁宽度及骨小梁数目均增加,且DZ-L组小鼠骨小梁数目增加,以上差异均有统计学意义(P<0.05或P<0.01)。结论:DZ对维甲酸致小鼠骨质疏松具有一定的改善作用。
ABSTRACT: OBJECTIVE: To investigate improvement effect of Duzhong zhuanggu (DZ) pills on retinoic acid-induced osteoporosis in mice. METHODS: 72 KM mice were randomized into blank control group (normal saline), model group (normal saline), positive control group (Xianling gubao capsule, 0.50 g/kg), DZ pills high-dose, medium-dose and low-dose groups (1.90,0.95,0.48 g/kg, DZ-H, DZ-M, DZ-L groups), with 12 mice in each group. Except for blank control group, other groups were given retinoic acid intragastrically to induce osteoporosis model, and given relevant medicine intragastrically once a day, for 14 days. After last administration, body weight of mice was determined; the blood sample was collected from eyeball to determine the contents of serum calcium and phosphor; liver, spleen and thymus gland were collected to determine organ index; left femur was collected to determine bone wet weight, bone dry weight, length and diameter; right femur was collected to determine the area percentage, width and number of bone trabecula. RESULTS: Compared with blank control group, the body weight of mice in model group were decreased; the contents of serum calcium and phosphorus were increased; liver indexes and  spleen indexes were increased, while thymus indexes were decreased; bone wet weight, bone dry weight, length and diameter were decreased; area percentage, width and number of trabecular were decreased, with statistical significance (P<0.05 or P<0.01). Compared with model group, the contents of serum calcium were decreased in treatment groups, and the contents of serum phosphorus were decreased in positive control group, DZ-M and DZ-L groups. Thymus indexes were increased in positive control group, DZ-M and DZ-L groups, while liver indexes were decreased in positive control group; femur wet weight of positive control group as well as femur dry and wet weight, diameters of DZ-M were all increased. The percentage, width and number of bone trabecula were increased in positive control group, DZ-H and DZ-M group, and the number of bone trabecula was increased in DZ-L group, with statistical significance (P<0.05 or P<0.01). CONCLUSIONS: DZ pills have improvement effect on osteoporosis induced by retinoic acid in mice.
期刊: 2017年28卷第1期
作者: 刘明,宣振华,张永萍,刘毅,刘莉,王海洋
AUTHORS: LIU Ming,XUAN Zhenhua,ZHANG Yongping,LIU Yi,LIU Li,WANG Haiyang
关键字: 杜仲壮骨丸;维甲酸;骨质疏松模型;小鼠;股骨计量学指标
KEYWORDS: Duzhong zhuanggu pills; Retinoic acid; Osteoporosis model; Mice; Metrology index of femur
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