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基于复合酶的黄蜀葵花多糖提取工艺优化及多糖的活性研究
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| 篇名： | 基于复合酶的黄蜀葵花多糖提取工艺优化及多糖的活性研究 |
| TITLE： | Optimization of polysaccharide extraction from Abelmoschus manihot flowers using composite enzymes and a study on the activities of the polysaccharides |
| 摘要： | 目的 优化黄蜀葵花多糖的提取工艺，探讨多糖的抗氧化活性及保湿、吸湿活性。方法采用正交实验确定复合酶最优配比，通过单因素实验和Box-Behnken响应面法优化多糖的提取工艺并进行验证；采用2，2′-联氮-双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）法、Fenton-水杨酸显色法、邻苯三酚自氧化法、铁氰化钾还原法分别考察多糖对ABTS自由基、羟自由基、超氧阴离子自由基的清除率及总还原能力；通过称量法考察多糖的吸湿及保湿活性。结果复合酶最优配比为木瓜蛋白酶136000U、果胶酶125000U、纤维素酶5000U；最优提取工艺为酶解时间2.8h、酶解温度57.50℃、磷酸二氢钠-磷酸氢二钠缓冲盐溶液pH5.5、料液比1∶40。经3次实验验证，平均多糖提取率为23.595%，RSD为0.90%。所得多糖对ABTS自由基、羟自由基和超氧阴离子自由基的清除率及总还原能力分别为81.759%、87.110%、54.244%、1.129（多糖质量浓度分别为10、5、10、10mg/mL）。在43%相对湿度环境下放置12h时，多糖的保湿率和吸湿率分别为99.387%、100.478%；在83%相对湿度环境下放置12h时，多糖的保湿率和吸湿率分别为99.764%、100.455%。结论基于复合酶的黄蜀葵花多糖的最优提取工艺稳定、可行；该工艺下所得多糖具有良好的抗氧化、保湿及吸湿活性。 |
| ABSTRACT： | OBJECTIVE To optimize the extraction process of polysaccharides from Abelmoschus manihot flowers， and to investigate the antioxidant， moisturizing and hygroscopicity activities of the polysaccharides.METHODS The optimal ratio of composite enzymes was determined by orthogonal experiments， and the extraction process of polysaccharides was optimized by single-factor experiments and Box-Behnken response surface methodology， and validated. The scavenging rates of 2，2′-azino-bis （3-ethylbenzothiazoline-6-sulfonic acid） （ABTS） free radicals， hydroxyl free radicals， and superoxide anion free radicals， as well as the total reducing ability of the polysaccharides were investigated by the ABTS method， Fenton-salicylic acid colorimetric method， pyrogallol autoxidation method， and potassium ferricyanide reduction method， respectively. The moisturizing and hygroscopicity activities of the polysaccharides were evaluated by the weighing method.RESULTS The optimal ratio of composite enzymes was 136 000 U of papain， 125 000 U of pectinase，and 5 000 U of cellulase. The optimal extraction process was as follows： enzymatic hydrolysis time of 2.8 h， enzymatic hydrolysis temperature of 57.50 ℃， pH of the sodium dihydrogen phosphate-disodium hydrogen phosphate buffer was 5.5， and solid-to-liquid ratio of 1∶40. The results of three experiments showed that the average polysaccharides extraction rate of 23.595%， with an RSD of 0.90%. The scavenging rates of ABTS free radicals， hydroxyl free radicals， superoxide anion free radicals， and total reducing ability were 81.759%， 87.110%， 54.244%， and 1.129 （the mass concentrations of polysaccharides were 10、 5、 10， and 10 mg/mL， respectively）， respectively. At relative humidity of 43% for 12 h， the moisturizing rate and hygroscopicity rate of polysaccharides were 99.387% and 100.478%， respectively； at relative humidity of 83% for 12 h， they were 99.764% and 100.455%， respectively.CONCLUSIONS The optimal extraction process of polysaccharides from A.manihot flowers based on the composite enzymes is stable and feasible； the polysaccharides obtained under this process have good antioxidant， moisturizing， and hygroscopicity activities. |
| 期刊： | 2026年第37卷第18期 |
| 作者： | 刘鸽；罗成；柴慧芳；李彦；鹿田 |
| AUTHORS： | LIU Ge，LUO Cheng，CHAI Huifang，LI Yan，LU Tian |
| 关键字： | 黄蜀葵花;多糖;复合酶;响应面法;抗氧化;保湿;吸湿 |
| KEYWORDS： | Abelmoschus manihot flowers ;Polysaccharides;composite enzymes;Response surface methodology;antioxidant activity;Moisturizing;hygroscopicity |
| 阅读数： | 1 次 |
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