返回 
加入收藏
不同砂炒时间钩吻炮制品的颜色、成分、抗肿瘤活性变化及相关性研究
x
请在关注微信后，向客服人员索取文件
| 篇名： | 不同砂炒时间钩吻炮制品的颜色、成分、抗肿瘤活性变化及相关性研究 |
| TITLE： | Study on changes and correlations of color，components and anti-tumor activity of processed products of Gelsemium elegans at different sand-frying durations |
| 摘要： | 目的 探究钩吻砂炒过程中颜色、成分、抗肿瘤活性的变化及相关性，为砂炒钩吻饮片的“减毒存效”和质量控制提供参考。方法以钩吻生品和分别经砂炒15、30、75、105、120s后的钩吻炮制品为检测样品，测定其色度值以及总生物碱和7种主要活性成分（钩吻素甲、胡蔓藤碱丙、钩吻素子、钩吻素己等）的含量，考察其体外抗肿瘤活性，并分析其颜色-成分含量、总生物碱含量-抗肿瘤活性的关联性。结果砂炒炮制后钩吻样品的颜色总体变暗，砂炒75s后样品的总色差值（ΔE*）均大于20。在砂炒初期（15～30s），钩吻炮制品中总生物碱含量较生品大幅降低；在砂炒后期（75～105s），钩吻炮制品中总生物碱含量的下降趋势变缓并趋于稳定。各炮制品中钩吻素己、胡蔓藤碱乙等的含量在砂炒30s时急剧下降，钩吻素子、常绿钩吻碱等的含量在砂炒75s后大幅降低（约降低80%）并逐渐趋于稳定。各炮制品对HepG2细胞增殖的抑制活性随炒制时间的延长而减弱。相关性分析结果显示，颜色亮度、黄蓝色度与除钩吻素甲外的其余活性成分和总生物碱的含量均呈显著正相关（P＜0.05或P＜0.01）；红绿色度与钩吻素子、常绿钩吻碱、钩吻绿碱和胡蔓藤碱乙的含量均呈显著正相关（P＜0.05或P＜0.01）；ΔE*与除钩吻素甲外的其余活性成分和总生物碱的含量，以及总生物碱含量与样品对HepG2细胞的半数抑制浓度均呈显著负相关（P＜0.05或P＜0.01）。结论随砂炒时间的延长，钩吻炮制品色泽逐渐趋于棕褐色，7种活性成分和总生物碱含量逐步降低，抗HepG2细胞活性整体呈下降趋势；砂炒75s是上述变化的关键时间转折点。 |
| ABSTRACT： | OBJECTIVE To explore the changes and correlations of color， components and anti-tumor activity of Gelsemium elegans during sand-frying processing， so as to provide a reference for the toxicity-attenuating and efficacy-preserving processing as well as quality control of sand-fried G. elegans decoction pieces.METHODS Raw G.elegans and its processed products prepared by sand-frying for 15， 30， 75， 105 and 120 s were taken as test samples. The chromatic values， contents of total alkaloids and seven main active components （gelsemine， humantenine， koumine， gelsenicine， etc.） were determined. Their in vitro anti-tumor activity was investigated. The correlations between color and component contents， as well as between total alkaloid content and anti-tumor activity were analyzed.RESULTS The color of G.elegans samples generally darkened after sand-frying， and the total color difference （Δ E * ） of samples sand-fried for 75 s was greater than 20. At the initial stage of sand-frying （15-30 s）， the total alkaloid content in processed products decreased sharply compared with the raw material； at the later-stage of sand-frying （75-105 s）， the declining rate of total alkaloid content slowed down and tended to be stable. The contents of gelsenicine and humantenidine in processed products dropped drastically at 30 s of sand-frying， while the contents of koumine and sempervirine decreased substantially （by about 80%） after 75 s of sand-frying and then gradually tended to be stable. The anti-HepG2 cell proliferation activity of each processed product decreased with the extension of frying time. Correlation analysis demonstrated that color brightness and yellow-blue chromaticity were significantly positively correlated with the contents of total alkaloids and all determined active components except gelsemine （ P ＜0.05 or P ＜0.01）； red-green chromaticity was significantly positively correlated with the contents of koumine， sempervirine， gelsemivirine and humantenidine （ P ＜0.05 or P ＜0.01）； Δ E * was significantly negatively correlated with the contents of total alkaloids and all determined active components except gelsemine. Besides， total alkaloid content was significantly negatively correlated with the half maximal inhibitory concentration of the samples on HepG2 cells （ P ＜0.05 or P ＜0.01）.CONCLUSIONS With the prolongation of sand-frying time， the color of sand-fried G.elegans decoction pieces gradually turned brownish-brown， the contents of seven active components and total alkaloids decreased progressively， and its anti-HepG2 cell activity showed an overall downward trend. Sand-frying for 75 s serves as a critical turning-point for the above-mentioned changes. |
| 期刊： | 2026年第37卷第17期 |
| 作者： | 吴昊；陈丽松；黄成龙；王月灵；陈丽蓉；王文义；王英豪；李德森；吴水生；陈莹 |
| AUTHORS： | WU Hao，CHEN Lisong，HUANG Chenglong， WANG Yueling，CHEN Lirong，WANG Wenyi，WANG Yinghao，LI Desen，WU Shuisheng，CHEN Ying |
| 关键字： | 钩吻；砂炒钩吻；色度值；活性成分；抗肿瘤活性；总生物碱；相关性分析；减毒存效 |
| KEYWORDS： | sand-fried Gelsemium elegans ;chromatic value;active components;anti-tumor activity;total alkaloids;correlation analysis;toxicity-attenuating and efficacy-preserving |
| 阅读数： | 0 次 |
| 本月下载数： | 0 次 |
* 注：未经本站明确许可，任何网站不得非法盗链资源下载连接及抄袭本站原创内容资源!在此感谢您的支持与合作！
