返回 
加入收藏
通关藤饮片中两种特异性C21甾体皂苷含量测定方法的建立
x
请在关注微信后，向客服人员索取文件
| 篇名： | 通关藤饮片中两种特异性C21甾体皂苷含量测定方法的建立 |
| TITLE： | Establishment of content determination method for two specific C21‑steroid saponins in Marsdenia tenacissima decoction pieces |
| 摘要： | 目的 建立通关藤饮片中两种特异性C21甾体皂苷——11-O-异丁酰基-12-O-乙酰基通关藤苷元B-3-O-茯苓二糖基（化合物T）与17β-通关藤苷元B的含量测定方法。方法采用超高效液相色谱-串联质谱联用法（UPLC‑MS/MS），用XSelectHSST3XP色谱柱，以0.1%甲酸水-甲醇为流动相进行梯度洗脱，流速为0.3mL/min，进样量为5μL，柱温为40℃；质谱采用电喷雾离子源，以多反应监测模式进行正/负离子采集。结果化合物T和17β-通关藤苷元B的质量浓度分别在10～200、20～200ng/mL范围内与峰面积呈良好线性关系，r分别为0.9931、0.9986；精密度、重复性、准确度及稳定性的RSD均小于4.5%（n＝5～6）；在3批饮片中的含量分别为94.0～102.8、1705.4～1736.2μg/g。结论所建立的方法可用于通关藤饮片中上述两种特异性C21甾体皂苷的含量测定。 |
| ABSTRACT： | OBJECTIVE To establish a content determination method for two specific C21‑steroid saponins namely 3- O - β -allopyranosyl-（1→4）- β -oleandropyranosyl-11- O -isobutyryl-12- O -acetyl tenacigenin B（compound T） and 17 β -tenacigenin B in Marsdenia tenacissima decoction pieces.METHODS Ultra‑high‑performance liquid chromatography‑tandem mass spectrometry （UPLC‑MS/MS） was adopted. An XSelect HSS T3 XP chromatographic column was used. Gradient elution was performed with 0.1% formic acid aqueous solution-methanol as the mobile phase. The flow rate was 0.3 mL/min， the injection volume was 5 μL， and the column temperature was 40 ℃. An electrospray ionization source was applied for mass spectrometry， and multiple reaction monitoring mode was used for positive‑ and negative‑ion acquisition.RESULTS The linear relationships of compound T and 17 β -tenacigenin B were good within the concentration ranges of 10-200 ng/mL and 20-200 ng/mL， with r values of 0.993 1 and 0.998 6， respectively； the RSD values of precision， repeatability， accuracy， and stability were all below 4.5% （ n ＝5-6）. Their contents in three batches of decoction pieces were 94.0-102.8 μg/g and 1 705.4-1 736.2 μg/g， respectively.CONCLUSIONS The established method can be applied for the content determination of the two specific C21‑steroid saponins mentioned above in M. tenacissima decoction pieces. |
| 期刊： | 2026年第37卷第17期 |
| 作者： | 王祁民；鞠倩；范理菊；李颖；马银玲；董占军 |
| AUTHORS： | WANG Qimin，JU Qian，FAN Liju，LI Ying，MA Yinling，DONG Zhanjun |
| 关键字： | 通关藤；11-O-异丁酰基-12-O-乙酰基通关藤苷元B-3-O-茯苓二糖基；17β-通关藤苷元B；含量测定 |
| KEYWORDS： | 3- O - β -allopyranosyl-（1→4）- β -oleandropyranosyl-11- O -isobutyryl-12- O -acetyl tenacigenin B;17 β -tenacigenin B;content determination |
| 阅读数： | 0 次 |
| 本月下载数： | 0 次 |
* 注：未经本站明确许可，任何网站不得非法盗链资源下载连接及抄袭本站原创内容资源!在此感谢您的支持与合作！
