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红花多糖调节Gas6/Axl信号通路对大鼠缺血性脑卒中的影响
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| 篇名： | 红花多糖调节Gas6/Axl信号通路对大鼠缺血性脑卒中的影响 |
| TITLE： | Effects of safflower polysaccharide on ischemic stroke in rats by regulating the Gas6/Axl signaling pathway |
| 摘要： | 目的 探讨红花多糖（SPS）调节生长停滞特异性蛋白6（Gas6）/Axl受体酪氨酸激酶（简称为“Axl”）信号通路对大鼠缺血性脑卒中（IS）的影响。方法采用改良线栓法构建大脑中动脉闭塞IS模型大鼠，再随机分为模型（Model）组，SPS低、高剂量（SPS-L、SPS-H，50、100mg/kg）组、中西药结合（SPS-H+Nim，SPS100mg/kg+Nim15mg/kg）组，每组10只。另取10只大鼠作为假手术（Sham）组。各组大鼠灌胃相应剂量的药物或等体积生理盐水，连续干预14d。给药结束后24h，评估各组大鼠的神经功能；观察海马组织形态学变化，检测血清中白细胞介素6（IL-6）、IL-1β、肿瘤坏死因子α（TNF-α）含量，脑梗死面积百分比，神经元凋亡率和海马组织中丙二醛（MDA）、超氧化物歧化酶（SOD）、脑源性神经营养因子（BDNF）含量以及Gas6、磷酸化Axl（p-Axl）、Axl、B细胞淋巴瘤2（Bcl-2）、Bcl-2相关X蛋白（Bax）的蛋白相对表达量。结果与Model组比较，SPS-L组、SPS-H组和SPS-H+Nim组大鼠神经功能显著改善；血清中IL-6、TNF-α、IL-1β含量和脑梗死面积百分比、神经元凋亡率以及海马组织中MDA含量、p-Axl及Bax蛋白相对表达量均显著降低（P＜0.05或P＜0.01），海马组织中SOD、BDNF含量，Gas6、Axl及Bcl-2蛋白相对表达量均显著升高（P＜0.05或P＜0.01）；海马组织缺血性损伤均有不同程度改善。结论SPS可能通过激活Gas6/Axl信号通路，降低IS大鼠神经炎症、氧化应激和神经元凋亡，减轻神经损伤，改善神经功能。 |
| ABSTRACT： | OBJECTIVE To investigate the effects of safflower polysaccharide （SPS） on ischemic stroke （IS） in rats by regulating the growth arrest-specific protein 6 （Gas6）/Axl receptor tyrosine kinase （Axl） signaling pathway. METHODS The modified suture-occluded method was employed to establish a rat model of middle cerebral artery occlusion （MCAO） for IS. The rats were then randomly divided into the model （Model） group， SPS low- and high-dose （SPS-L， SPS-H， 50 and 100 mg/kg） groups， and integrated traditional Chinese and Western medicine （SPS-H+Nim， SPS 100 mg/kg+Nim 15 mg/kg） group， with 10 rats in each group. Another 10 rats were selected as the sham operation （Sham） group. Rats in each group were administered corresponding doses of medication or an equal volume of normal saline intragastrically， with continuous intervention for 14 days. The neurological function of rats in each group was evaluated 24 hours after drug administration. The morphological changes in hippocampal tissue were observed. The contents of interleukin-6 （IL-6）， IL-1β， and tumor necrosis factor-α （TNF-α） in serum， the percentage of cerebral infarction area， the neuronal apoptosis rate， as well as the contents of malondialdehyde （MDA）， superoxide dismutase （SOD）， and brain-derived neurotrophic factor （BDNF） in hippocampal tissue， and the relative protein expression levels of Gas6， phosphorylated Axl （p-Axl）， Axl， B-cell lymphoma-2 （Bcl-2）， and Bcl-2 associated X protein （Bax） were measured. RESULTS Compared with Model group， the neurological function of rats in SPS-L group， SPS-H group and SPS-H+Nim group improved significantly， while serum contents of IL-6， TNF-α， and IL-1β， the percentage of cerebral infarction area， the apoptotic rate of neurons as well as MDA content and relative protein expression of p-Axl and Bax were decreased significantly （ P ＜0.05 or P ＜0.01）. The contents of SOD and BDNF， as well as the relative protein expression of Gas6， Axl， and Bcl-2 in hippocampal tissue， were significantly increased （ P ＜0.05 or P ＜0.01）， and the ischemic injury in hippocampal tissue imp roved to varying degrees. CONCLUSIONS SPS can reduce neuroinflammation， oxidative stress， and neuronal apoptosis in IS rats， alleviate nerve injury， and improve neurological function， which may be achieved by activating the Gas6/Axl signaling pathway. |
| 期刊： | 2026年第37卷第08期 |
| 作者： | 陈彩凤；陆韵薇；李建宇 |
| AUTHORS： | CHEN Caifeng，LU Yunwei，LI Jianyu |
| 关键字： | 缺血性脑卒中；红花多糖；Gas6/Axl信号通路；神经炎症；神经元凋亡 |
| KEYWORDS： | ischemic stroke； safflower polysaccharide； |
| 阅读数： | 1 次 |
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