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加减理冲汤通过Fas/FasL途径及caspase凋亡途径对裸鼠移植瘤细胞凋亡的作用机制研究
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| 篇名： | 加减理冲汤通过Fas/FasL途径及caspase凋亡途径对裸鼠移植瘤细胞凋亡的作用机制研究 |
| TITLE： | Mechanism of modified Lichong decoction on apoptosis of transplanted tumor cells in nude mice via Fas/FasL pathway and caspase apoptosis pathway |
| 摘要： | 目的 探讨加减理冲汤（MLCD）对裸鼠移植瘤细胞凋亡的作用及机制。方法培养胃癌AGS细胞并构建裸鼠移植瘤模型；将裸鼠分为模型组以及MLCD低、中、高剂量组（150、300、600mg/kg），连续灌胃蒸馏水/相应药液，每日1次，连续4周。每6d测量1次裸鼠移植瘤的大小并计算移植瘤体积；给药结束后处死裸鼠并分离移植瘤组织，检测移植瘤组织中乳酸脱氢酶（LDH）、活性氧（ROS）含量并评估线粒体膜电位变化；观察移植瘤组织的病理形态变化；检测移植瘤组织中胱天蛋白酶（caspase）-3、cas‐pase-8、caspase-9酶活性，以及Fas、Fas配体（FasL）蛋白表达和caspase-3、caspase-8、caspase-9、Fas、FasLmRNA表达。结果与模型组比较，MLCD各剂量组裸鼠移植瘤体积均有不同程度缩小，LDH、ROS含量以及caspase-3、caspase-8、caspase-9酶活性均显著升高/增强，线粒体膜电位显著降低，Fas、FasL蛋白表达以及caspase-3、caspase-8、caspase-9、Fas、FasLmRNA表达均显著上调，以上指标大部分差异有统计学意义（P＜0.05或P＜0.01）。病理结果显示，随着MLCD给药剂量的增加，移植瘤组织中细胞的紧密程度逐渐下降，出现松散、碎片逐渐增多等典型的细胞凋亡形态学特征。结论MLCD能诱导裸鼠移植瘤细胞凋亡，其作用机制可能与激活Fas/FasL途径及caspase凋亡途径有关。 |
| ABSTRACT： | OBJECTIVE To investigate the effect and mechanism of modified Lichong decoction （MLCD） on the apoptosis of transplanted tumor cells in nude mice. METHODS Human gastric cancer AGS cells were cultured， and a nude mice transplanted tumor model was established. The nude mice were divided into the model group and MLCD low-， medium- and high-dose groups （150， 300， 600 mg/kg）. They were given distilled water or the corresponding drug solution by gavage once daily for four consecutive weeks. The size of transplanted tumors in nude mice was measured every six days， and the tumor volume was calculated. After the medication， the nude mice were sacrificed， and the transplanted tumor tissues were isolated. The contents of lactate dehydrogenase （LDH） and reactive oxygen species （ROS） in the transplanted tumor tissues were detected， and the changes in mitochondrial membrane potential were assessed. The pathological morphological changes were observed. The enzymatic activities of caspase-3， caspase-8， and caspase-9， as well as protein expressions of Fas and FasL and mRNA expressions of caspase-3， caspase-8， caspase-9， Fas and FasL in the transplanted tumor tissues， were detected. RESULTS Compared with the model group， the volume of transplanted tumors in nude mice from all MLCD dose groups was reduced to varying degrees. The contents of LDH and ROS， as well as the enzymatic activities of caspase-3， caspase-8 and caspase-9， were significantly increased/enhanced. The mitochondrial membrane potential was significantly decreased. The protein expressions of Fas and FasL， and the mRNA expressions of caspase-3， caspase-8， caspase-9， Fas and FasL were significantly up-regulated. Most of these differences were statistically significant （ P ＜0.05 or P ＜0.01）. Pathological results showed that with increasing doses of MLCD， the cellular density in the transplanted tumor tissues gradually decreased， and typical morphological features of apoptosis， such as loosening and increasing fragmentation， became more prominent. CONCLUSIONS MLCD can induce apoptosis in transplanted tumor cells of nude mice， and its mechanism may be related to the activation of the Fas/FasL pathway and the caspase apoptotic pathway. |
| 期刊： | 2026年第37卷第07期 |
| 作者： | 施国善；郝铁霞；陈哲；李旭；马立威 |
| AUTHORS： | SHI Guoshan，HAO Tiexia，CHEN Zhe，LI Xu，MA Liwei |
| 关键字： | 加减理冲汤；AGS细胞；caspase凋亡途径；Fas/FasL途径；细胞凋亡 |
| KEYWORDS： | modified Lichong decoction； AGS cells； caspase apoptotic pathway； Fas/FasL pathway； cell apoptosis |
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