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基于HPLC指纹图谱结合一测多评法的漆姑草药材质量控制研究
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| 篇名： | 基于HPLC指纹图谱结合一测多评法的漆姑草药材质量控制研究 |
| TITLE： | Quality control of Sagina japonica by HPLC fingerprint combined with quantitative analysis of multi-components by single-marker |
| 摘要： | 目的 建立漆姑草药材的高效液相色谱（HPLC）指纹图谱，并建立同时测定漆姑草中6种成分含量的一测多评法，以期为该药材的质量控制提供参考。方法采用HPLC法，根据《中药色谱指纹图谱相似度评价系统》建立12批（编号S1～S12）漆姑草的指纹图谱并进行相似度评价和共有峰指认，然后对12批药材进行聚类分析（CA）和主成分分析（PCA）。以维采宁-2作为内参物，采用一测多评法测定对羟基肉桂酸、apigenin-6-C-arabinoside-8-C-glucoside、异荭草素、牡荆素、20-hydroxyecdysone的含量，并与外标法测定结果进行比较。结果12批漆姑草药材的相似度为0.828～0.998；共标定了17个共有峰，并指认了6个共有峰，其中5号峰为维采宁-2、7号峰为对羟基肉桂酸、10号峰为apigenin-6-C-arabinoside-8-C-glucoside、11号峰为异荭草素、13号峰为牡荆素、15号峰为20-hydroxyecdysone。CA结果显示，样品S1～S5、S7、S9～S11聚为一类，S6为一类，S8和S12聚为一类；PCA结果显示，前4个主成分的累计贡献率为89.430%。一测多评法测定结果显示，对羟基肉桂酸、apigenin-6-C-arabinoside-8-C-glucoside、异荭草素、牡荆素、20-hydroxyecdysone的含量分别为0.0174～0.2694、0.5688～4.2403、0.5032～5.0403、0.0240～0.1320、2.5513～4.8811mg/g，与外标法所测结果差异无统计学意义（P＞0.05）。结论本研究所建立的HPLC指纹图谱和一测多评法，可为漆姑草药材的质量评价和质量控制提供参考。 |
| ABSTRACT： | OBJECTIVE To establish the high-performance liquid chromatography （HPLC） fingerprint of Sagina japonica ， and to establish a quantitative analysis of multi-components by single-marker （QAMS） method for simultaneous determination of six componen ts in S. japonica ， aiming to provide references for the quality control of this medicinal herb. METHODS HPLC method was used to establish the fingerprints of 12 batches （No. S1-S12） of S . japonica according to Similarity Evaluation System of Chromatographic Fingerprint of Traditional Chinese Medicine . The similarity evaluation and identification of common peaks were conducted， followed by cluster analysis （CA） and principal component analysis （PCA） for 12 batches of samples. Using vicenin-2 as internal reference， the contents of p-hydroxy cinnamic acid， apigenin-6-C-arabinoside-8-C-glucoside， isoorientin， vitexin and 20-hydroxyecdysone were determined by QAMS method. The results were then compared with those obtained by the external standard method. RESULTS The similarities of HPLC fingerprints for 12 batches of S . japonica ranged from 0.828-0.998. A total of 17 common peaks were calibrated， and 6 common peaks were identified. Specifically， peak 5 was identified as vicenin-2， peak 7 as p-hydroxycinnamic acid， peak 10 as apigenin-6-C-arabinoside-8-C-glucoside， peak 11 as isoorientin， peak 13 as vitexin， and peak 15 as 20-hydroxyecdysone. The results of CA showed that S1-S5， S7 and S9-S11 were clustered into one category， S6 was clustered into one category， and S8 and S12 were clustered into one category. The results of PCA revealed that the accumulative contribution rate of the four main components was 89.430%. The content ranges measured by QAMS method for p-hydroxy cinnamic acid， apigenin-6-C-arabinoside-8-C-glucoside， isoorientin， vitexin and 20-hydroxyecdysone were 0.017 4-0.269 4， 0.568 8-4.240 3， 0.503 2-5.040 3， 0.024 0-0.132 0 and 2.551 3-4.881 1 mg/g， respectively. There was no significant difference in the contents of components measured between QAMS method and the external standard method （ P ＞0.05）. CONCLUSIONS The established HPLC fingerprint and QAMS method can be used for quality evaluation and quality control of S . japonica. |
| 期刊： | 2026年第37卷第07期 |
| 作者： | 刘俊宏；黎雪；张美秦；胡涵；柏春梅；刘春花；李勇军 |
| AUTHORS： | LIU Junhong，LI Xue，ZHANG Meiqin，HU Han，BAI Chunmei，LIU Chunhua，LI Yongjun |
| 关键字： | 漆姑草；指纹图谱；一测多评法；质量控制；质量评价；高效液相色谱法 |
| KEYWORDS： | Sagina japonica； fingerprint； quantitative analysis of multi-components by single-marker； quality control； quality |
| 阅读数： | 1 次 |
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