返回 
加入收藏
中康酸联合乳酸钠林格注射液延长高原失血性休克大鼠黄金救治时间窗的作用及复苏效果
x
请在关注微信后，向客服人员索取文件
| 篇名： | 中康酸联合乳酸钠林格注射液延长高原失血性休克大鼠黄金救治时间窗的作用及复苏效果 |
| TITLE： | Effect of mesaconate combined with LR on prolonging the golden treatment time window and its resuscitation efficacy for hemorrhagic shock rats under high-altitude conditions |
| 摘要： | 目的 观察内源性代谢物中康酸联合乳酸钠林格注射液（LR）延长高原条件下失血性休克大鼠黄金救治时间窗的作用及复苏效果。方法将大鼠分为休克组、LR组和5、20、50mg/kg中康酸+LR组，每组20只，以考察加用中康酸对黄金救治时间窗的影响。各组大鼠以低压缺氧舱饲养+脾动脉离断的方式构建高原条件下非控制性失血性休克模型后，休克组大鼠不进行复苏，LR组和中康酸+LR组大鼠以LR或中康酸联合LR进行低压复苏，观察各组大鼠的血压控制情况、输液量、失血率和存活情况。进一步将大鼠分为正常组、休克组和中康酸（50mg/kg）+LR组，每组10只或20只，以考察加用中康酸延长黄金救治时间窗后的复苏效果。除正常组外，其余各组同法构建高原条件下非控制性失血性休克模型。休克组大鼠不进行复苏；中康酸+LR组大鼠以中康酸联合LR进行低压复苏至3h，结扎脾动脉止血，记录输液量和失血率，随后按2倍失血量进行LR复苏。检测中康酸+LR组大鼠不同时间点的血气指标，观察各组大鼠的存活情况、舌下微循环灌注功能相关指标、肝/肾血流量、重要器官功能相关指标和肺/脑含水量。结果单纯LR复苏能使休克大鼠平均动脉压（MAP）在50～60mmHg内维持约1h，中康酸与LR联合应用可使其MAP在50～60mmHg稳定维持3h以上，且50mg/kg中康酸+LR组大鼠的输液量、失血率均较LR组显著减少（P＜0.05）。LR组大鼠低压维持至1h后，存活率为52.94%，无大鼠存活超过2h；5、20、50mg/kg中康酸+LR组大鼠低压维持至1h后，存活率均超过80%；20、50mg/kg中康酸+LR组大鼠低压维持至3h后，存活率仍超过70%。经中康酸联合LR彻底复苏后，该组大鼠的72h存活率为43.75%，其血气指标均较休克期末显著改善（P＜0.05）。与休克组比较，中康酸+LR组大鼠彻底复苏后的舌下微循环相关指标和肝、肾血流量均显著恢复（P＜0.05），心、肝、肾功能相关指标和肺含水量均显著降低（P＜0.05）。结论中康酸联合LR可显著延长高原条件下失血性休克大鼠黄金救治时间窗，改善其血气指标、舌下微血管循环灌注及肝/肾血流量，减轻重要器官功能损伤和肺水肿，提高休克大鼠的存活率。 |
| ABSTRACT： | OBJECTIVE To observe the effects of endogenous metabolite mesaconate combined with Sodium lactate Ringer’s injection （LR） on prolonging the golden treatment time window and its resuscitation efficacy in rats with hemorrhagic shock under high-altitude conditions. METHODS Rats were divided into the shock group， LR group， and 5， 20， 50 mg/kg mesaconate+LR groups， with 20 rats in each group， to investigate the effect of additional use of mesaconate on the golden treatment time window. After establishing a model of uncontrolled hemorrhagic shock under high-altitude conditions in all groups by housing in a hypobaric hypoxia chamber combined with splenic artery transection， rats in the shock group received no resuscitation， while rats in the LR group and mesaconate+LR groups underwent low-pressure resuscitation with LR or mesaconate combined with LR. Blood pressure control， fluid infusion volume， blood loss rate and survival status were observed in each group. Rats were further divided into the normal group， shock group and mesaconate （50 mg/kg）+LR group， with 10 or 20 rats in each group， to evaluate the resuscitation effects after extending the golden treatment time window by additionally using mesaconate. Except for the normal group， the other groups underwent the same procedure to establish an uncontrolled hemorrhagic shock model under high-altitude conditions. Rats in the shock group received no resuscitation. In the mesaconate+LR group， after 3 h of low-pressure resuscitation， bleeding control was performed by ligation of the spleen artery， and the infusion volume and blood loss rate were recorded； subsequently， the rats received LR resuscitation with twice the volume of blood loss. Then， blood gas indicators of the mesaconate+LR group were measured at different time points. Survival rates， indicators related to sublingual microcirculatory perfusion， liver and kidney blood flow， indicators related to the function of vital organs， and lung and brain water content were observed in all groups. RESULTS LR infusion alone could effectively maintain mean arterial pressure （MAP） within 50-60 mmHg for approximately 1 h. The administration of mesaconate combined with LR during hypotensive resuscitation could maintain MAP within 50-60 mmHg for over 3 h， with significantly reduced fluid infusion volume and blood loss rate in 50 mg/kg mesaconate+LR group， compared to the LR group （ P ＜0.05）. In the LR group， rats maintained low pressure for up to 1 hour with a survival rate of 52.94%， and no rats survived beyond 2 h. In the 5， 20 and 50 mg/kg mesaconate+LR groups， rats maintained low pressure for up to 1 h with a survival rate exceeding 80%； in the 20 and 50 mg/kg mesaconate+LR groups， rats maintained low pressure for up to 3 h with a survival rate exceeding 70%. After complete resuscitation with mesaconate combined with LR， the 72 h survival rate of rats was 43.75%， and significant improvements in blood gas parameters were observed compared to the end of the shock phase （ P ＜0.05）. Compared to the shock group， the mesaconate+LR group showed significant recovery in sublingual microcirculatory indicators， and liver/kidney blood flow after complete resuscitation （ P ＜0.05）， with significant reductions in heart， liver and kidney function-related indicators and lung water content （ P ＜0.05）. CONCLUSIONS Mesaconate combined with LR significantly extends the golden treatment time window for hemorrhagic shock in rats under high-altitude conditions， improves blood gas parameters， sublingual microcirculatory perfusion， and liver/kidney blood flow， mitigates vital organ impairment and pulmonary edema， and increases the survival rate of shocked rats. |
| 期刊： | 2026年第37卷第06期 |
| 作者： | 周远群；向鑫明；欧阳杏楠；张杰；李清晖；刘良明；李涛 |
| AUTHORS： | ZHOU Yuanqun，XIANG Xinming，OUYANG Xingnan，ZHANG Jie，LI Qinghui，LIU Liangming，LI Tao |
| 关键字： | 中康酸；乳酸钠林格注射液；高原条件；失血性休克；早期救治；液体复苏；黄金救治时间窗；复苏效果 |
| KEYWORDS： | mesaconate； Sodium lactate Ringer’s injection； high-altitude conditions； hemorrhagic shock； fluid resuscitation； |
| 阅读数： | 0 次 |
| 本月下载数： | 0 次 |
* 注：未经本站明确许可，任何网站不得非法盗链资源下载连接及抄袭本站原创内容资源!在此感谢您的支持与合作！
