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积雪草苷对兔耳增生性瘢痕形成的抑制作用及机制
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| 篇名： | 积雪草苷对兔耳增生性瘢痕形成的抑制作用及机制 |
| TITLE： | Inhibitory effect and mechanism of asiaticoside on hypertrophic scar formation in rabbit ears |
| 摘要： | 目的 基于Wnt/β-catenin通路，研究积雪草苷对兔耳增生性瘢痕形成的抑制作用及机制。方法构建兔耳增生性瘢痕模型，将造模成功的兔分为模型组（生理盐水）、积雪草苷低剂量组（12mg/kg）、积雪草苷高剂量组（24mg/kg）、阳性对照组（醋酸曲安奈德40mg/kg），另设不造模的对照组（生理盐水），每组8只。各组兔于瘢痕内基底部注射相应药液/生理盐水，每天给药1次，连续28d。末次给药结束后，观察兔耳增生性瘢痕外观并测定增生性瘢痕指数，检测兔耳增生性瘢痕组织中炎症因子水平[白细胞介素6（IL-6）、IL-10]，观察兔耳增生性瘢痕组织病理学形态，检测兔耳增生性瘢痕组织中Ⅰ型胶原（COLⅠ）、COLⅢ、Wnt4、β-catenin蛋白表达水平。结果与对照组比较，模型组兔耳出现增生性瘢痕，角质层变厚，真皮组织中胶原纤维异常沉积且排列较乱；增生性瘢痕指数、IL-6水平、成纤维细胞密度、胶原纤维面密度和COLⅠ、COLⅢ的平均光密度值以及Wnt4、β-catenin蛋白的表达水平均显著升高（P＜0.05），IL-10水平显著降低（P＜0.05）。与模型组比较，积雪草苷低、高剂量组兔耳增生性瘢痕软化，角质层变薄，上述定量指标均显著逆转（P＜0.05）。结论积雪草苷可抑制兔耳增生性瘢痕形成，减轻瘢痕组织炎症反应，其作用机制可能与抑制Wnt/β-catenin通路的激活有关。 |
| ABSTRACT： | OBJECTIVE To explore the inhibitory effect and its mechanism of asiaticoside on hypertrophic scar formation in rabbit ears based on the Wnt/ β -catenin pathway. METHODS The hypertrophic scar model in rabbit ears was established. Model rabbits were divided into the model group （normal saline）， asiaticoside low-dose group （12 mg/kg）， asiaticoside high-dose group （24 mg/kg）， and positive control group （tri amcinolone acetonide acetate 40 mg/kg）； additionally， a non-modeled control group （normal saline） was set up. There were 8 rabbits in each group. Rabbits in each group were injected with the corresponding drug solution/normal saline at the base within the scar area once daily for 28 consecutive days. After the last medication， the appearance of hypertrophic scars in rabbit ears was observed， and the hypertrophic scar index was measured； the levels of inflammatory factors [interleukin-6 （IL-6） and IL-10 ] in hypertrophic scar tissue of rabbits were determined； histopathological morphology of hypertrophic scar tissues in rabbit ears was observed， and the protein expression levels of collagen type Ⅰ （COLⅠ）， COLⅢ， Wnt4 and β -catenin in the hypertrophic scar tissues were detected. RESULTS Compared with control group， in the model group， the symptoms of hypertrophic scars were observed， the stratum corneum was thicker， and there was abnormal accumulation and disorganized arrangement of collagen. The hypertrophic scar index， IL-6 level， fibroblast density， area density of collagen fibers， mean optical density values of COLⅠ and COLⅢ， as well as the expression levels of Wnt4 and β -catenin proteins， were all significantly elevated （ P ＜0.05）， while the level of IL-10 was significantly decreased （ P ＜0.05）. Compared with the model group， the hypertrophic scars in asiaticoside low- and high-dose groups were significantly softened， with a thinned stratum corneum， and the above quantitative indicators were all significantly reversed （ P ＜0.05）. CONCLUSIONS Asiaticoside can inhibit the formation of hypertrophic scars in rabbit ears and alleviate the inflammatory response in scar tissue. Its mechanism of action may be related to the inhibition of activation of the Wnt/ β -catenin pathway. |
| 期刊： | 2026年第37卷第05期 |
| 作者： | 李其爱；周文娇；许秉诚 |
| AUTHORS： | LI Qiai，ZHOU Wenjiao，XU Bingcheng |
| 关键字： | 积雪草苷；增生性瘢痕；Wnt/β-catenin通路；炎症反应 |
| KEYWORDS： | asiaticoside； hypertrophic scar； Wnt/β-catenin |
| 阅读数： | 2 次 |
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