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基于组学技术的中医药干预腹泻型肠易激综合征机制研究进展
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| 篇名： | 基于组学技术的中医药干预腹泻型肠易激综合征机制研究进展 |
| TITLE： | Progress in mechanistic research on traditional Chinese medicine interventions for irritable bowel syndrome with diarrhea based on omics technologies |
| 摘要： | 腹泻型肠易激综合征（IBS-D）作为微生物群-肠-脑轴相关的典型疾病，其发病机制尚未完全明确，临床诊疗仍面临挑战。组学技术凭借高通量检测与系统性解析的优势，成为了解码中医药治疗IBS-D的复杂机制的关键工具。本文系统综述了转录组学、蛋白质组学、代谢组学、微生物组学及多组学整合技术在中医药干预IBS-D中的研究进展，发现在单一组学研究中，转录组学可通过RNA测序等技术，揭示中医药对IBS-D相关信号通路的调控机制；蛋白质组学可借助双向差异凝胶电泳、串联质谱标签等定量技术筛选出差异蛋白，阐明中医药治疗IBS-D的作用靶点；代谢组学可通过超高效液相色谱-四极杆-静电场轨道阱质谱、液相色谱-串联质谱等方法，发现中医药改善IBS-D代谢紊乱的相关通路；微生物组学可基于16SRNA基因测序，证实中医药能重塑肠道菌群结构，恢复肠道微生态平衡，从而改善IBS-D。多组学整合研究能进一步突破单一组学局限，整合转录组学、蛋白质组学、代谢组学、微生物组学等多方面信息，更加全面、系统地阐释中医药治疗IBS-D的复杂机制。未来应从中医证型分层临床研究、多组学整合验证机制、新兴组学挖掘新机制三方面推进IBS-D相关研究，为该病的精准诊疗提供更多创新思路。 |
| ABSTRACT： | Irritable bowel syndrome with diarrhea （IBS-D）， as a prototypical disorder involving the microbiota-gut-brain axis， remains poorly understood in terms of its pathogenesis， posing ongoing challenges for clinical diagnosis. Omics technologies， leveraging their high-throughput detection and systematic analysis advantages， has emerged as a critical tool for deciphering the complex mechanisms underlying traditional Chinese medicine （TCM） treatment of IBS-D. This systematic review summarizes the research progress of transcriptomics， proteomics， metabolomics， microbiomics， and multi-omics integration techniques in TCM interventions for IBS-D. In single-omics studies， transcriptomics using techniques like RNA-seq， reveals the regulatory mechanisms of TCM on IBS-related signaling pathways. Proteomics， leveraging quantitative technologies such as 2D-difference gel electrophoresis and tandem mass tag， identifies differentially expressed proteins and elucidates the action targets of TCM in treating IBS-D. Metabolomics， employing methods like UPLC-Q-TOF-MS and LC-MS/MS， discovers metabolic pathways regulated by TCM to improve metabolic disturbances in IBS-D. Microbiomics， based on 16S rRNA sequencing， confirms that TCM can reshape the gut microbiota structure and restore the intestinal microecological balance， thereby improving IBS-D. Multi-omics integration further overcomes the limitations of single-omics approaches by synthesizing information from transcriptomics， proteomics， metabolomics， and microbiomics， enabling a more comprehensive and systematic elucidation of the complex mechanisms underlying TCM treatment for IBS-D. In the future， research related to IBS-D should be advanced from three aspects： stratified clinical research based on TCM syndrome types， multi-omics integration verification mechanisms， and emerging omics to explore new mechanisms， providing more innovative ideas for the precise diagnosis and treatment of this disease. |
| 期刊： | 2026年第37卷第03期 |
| 作者： | 高山雪；马佳乐；彭龙；陈婕 |
| AUTHORS： | GAO Shanxue，MA Jiale，PENG Long，CHEN Jie |
| 关键字： | 肠易激综合征；中医药；肠道微生态；转录组学；蛋白质组学；代谢组学；微生物组学；多组学整合 |
| KEYWORDS： | irritable bowel syndrome； traditional Chinese medicine； gut microbiota； transcriptomics； proteomics； |
| 阅读数： | 10 次 |
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