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补阳还五汤及其有效部位改善非酒精性脂肪性肝病的作用及机制研究
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| 篇名： | 补阳还五汤及其有效部位改善非酒精性脂肪性肝病的作用及机制研究 |
| TITLE： | Ameliorative effects and mechanisms of Buyang huanwu decoction and its active fractions on non-alcoholic fatty liver disease |
| 摘要： | 目的 研究补阳还五汤（BYHWD）及其有效部位改善非酒精性脂肪性肝病的作用及机制。方法制备BYHWD及其醇沉、30%乙醇、50%乙醇、75%乙醇有效部位（即CC、30YC、50YC、75YC有效部位），以此灌胃大鼠并制备相应含药血清（同法制备空白血清和辛伐他汀含药血清）。将人L02肝细胞分为对照组、模型组、辛伐他汀含药血清组、BYHWD含药血清组、CC含药血清组、30YC含药血清组、50YC含药血清组、75YC含药血清组。除对照组外，其余各组均加入0.2mol/L油酸诱导24h，以建立脂质沉积模型，再加入相应20%含药血清/空白血清干预24h。观察细胞中脂质沉积情况，并计算脂滴面积占比；检测细胞中甘油三酯（TG）及氧化应激[丙二醛（MDA）、超氧化物歧化酶（SOD）]、肝功能[丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）]指标水平；检测细胞中AMP活化的蛋白质激酶（AMPK）/固醇调节元件结合蛋白1（SREBP-1）/甘油-3-磷酸酰基转移酶（GPAT）信号通路相关蛋白及mRNA表达水平。结果与对照组比较，模型组细胞脂肪变性严重，细胞中脂滴面积占比以及TG、ALT、AST、MDA水平和SREBP-1、GPATmRNA及其蛋白表达水平均显著升高（P＜0.05），细胞中SOD水平、AMPKmRNA表达水平和AMPK蛋白磷酸化水平均显著降低（P＜0.05）。与模型组比较，各含药血清组细胞脂肪变性均减轻，上述大部分定量指标水平均被显著逆转（P＜0.05）。结论BYHWD及其有效部位可通过调控AMPK/SREBP-1/GPAT信号通路，抑制氧化应激反应，减轻脂质沉积，从而发挥改善非酒精性脂肪性肝病的作用。 |
| ABSTRACT： | OBJECTIVE To investigate the effects and mechanisms of Buyang huanwu decoction （BYHWD） and its active fractions in ameliorating non-alcoholic fatty liver disease. METHODS BYHWD and its effective fractions obtained through ethanol precipitation， as well as 30% ethanol， 50% ethanol， and 75% ethanol fractions （namely， the CC effective fraction， 30YC effective fraction， 50YC effective fraction， and 75YC effective fraction）， were prepared. These preparations were administered to rats via intragastric administration to prepare corresponding drug-containing serum （blank serum and simvastatin-containing serum were prepared using the same protocol）. Human L02 hepatocytes were divided into control group， model group， simvastatin-containing serum group， BYHWD-containing serum group， CC-containing serum group， 30YC-containing serum group， 50YC-containing serum group， and 75YC-containing serum group. Except for the control group， other groups were given 0.2 mol/L oleic acid for 24 h to induce a lipid accumulation model， and then intervened with 20% drug-containing serum/blank serum for 24 h. The lipid deposition in cells was observed， and the proportion of lipid droplet area was calculated； the levels of triglycerides （TG） and indicators of oxidative stress [malondialdehyde （MDA）， superoxide dismutase （SOD）] as well as liver function [alanine amino- transferase （ALT）， aspartate amino-transferase （AST）] in cells were detected； protein and mRNA expressions of AMP-activated protein kinase （AMPK）/sterol regulatory element-binding protein-1 （SREBP-1）/glycerol-3-phosphate acyltransferase （GPAT） signaling pathway were also measured. RESULTS Compared with the control group， cells in the model group exhibited severe cellular steatosis， with a significantly increased proportion of lipid droplet area， as well as the elevated levels of TG， ALT， AST， and MDA in cells， along with significantly up-regulated mRNA and protein expression levels of SREBP-1 and GPAT （P＜0.05）. The level of SOD， mRNA expression of AMPK， as well as the protein phosphorylation level of AMPK were decreased significantly （P＜0.05）. Compared with the model group， cellular steatosis was alleviated in all drug-containing serum groups， and the levels of most of the aforementioned quantitative indicators were significantly reversed （P＜0.05）. CONCLUSIONS BYHWD and its active fractions can exert a therapeutic effect on improving non-alcoholic fatty liver disease by regulating the AMPK/SREBP-1/GPAT signaling pathway， inhibiting oxidative stress responses， and reducing lipid deposition. |
| 期刊： | 2026年第37卷第03期 |
| 作者： | 杨金彪；陈星彤；周云越；杨蕊红；王乔；薛爽；张玉昆；牛雯颖 |
| AUTHORS： | YANG Jinbiao， CHEN Xingtong，ZHOU Yunyue，YANG Ruihong，WANG Qiao，XUE Shuang，ZHANG Yukun，NIU Wenying |
| 关键字： | 补阳还五汤；非酒精性脂肪性肝病；脂质沉积；AMPK/SREBP-1/GPAT信号通路；氧化应激 |
| KEYWORDS： | Buyang huanwu decoction； non-alcoholic fatty liver disease； lipid deposition； AMPK/SREBP-1/GPAT signaling |
| 阅读数： | 2 次 |
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