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蠲哮汤改善支气管哮喘的机制研究
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| 篇名： | 蠲哮汤改善支气管哮喘的机制研究 |
| TITLE： | Study on the mechanism of Juanxiao decoction in improving bronchial asthma |
| 摘要： | 目的 基于核苷酸结合结构域富含亮氨酸重复序列和含热蛋白结构域受体3（NLRP3）炎症小体信号通路，探讨蠲哮汤改善支气管哮喘（简称“哮喘”）的潜在机制。方法将雌性SD大鼠随机分为正常组，模型组，蠲哮汤低、中、高剂量组（0.36、0.72、1.44g/kg，以生药量计），阳性对照组（醋酸地塞米松片，0.2mg/kg），每组10只。除正常组外，其余大鼠均按腹腔注射鸡卵清白蛋白+氢氧化铝联合雾化吸入鸡卵清白蛋白的方式构建哮喘模型。从实验第14天起，各组大鼠灌胃相应药液或生理盐水，每天1次，连续7d。末次给药后，检测各组大鼠肺功能指标（用力肺活量、第0.3秒用力呼气容积、呼气流量峰值）、血清炎症指标（白细胞介素1β、白细胞介素18）水平、支气管肺泡灌洗液中炎症细胞（淋巴细胞、嗜酸性粒细胞、中性粒细胞）百分比，观察其肺组织病理改变，并检测其肺组织中核因子κB（NF-κB）、NLRP3、胱天蛋白酶1（caspase-1）蛋白及mRNA的表达情况。结果与正常组相比，模型组大鼠可见肺泡壁增厚、炎症细胞浸润等病理改变；模型组和各药物组大鼠的各项肺功能指标均显著降低，炎症指标水平、炎症细胞百分比以及NF-κB、NLRP3、caspase-1蛋白及mRNA的表达均显著升高或上调（P＜0.05）。与模型组相比，蠲哮汤各剂量组大鼠的病理改变明显缓解，各定量指标均呈剂量依赖性改善（P＜0.05）。结论蠲哮汤可减轻哮喘大鼠的气道炎症反应，缓解肺功能损伤，改善炎症细胞浸润等病理改变；上述作用可能与抑制NLRP3炎症小体信号通路有关。 |
| ABSTRACT： | OBJECTIVE To investigate the potential mechanism by which Juanxiao decoction improves bronchial asthma （hereinafter referred to as “asthma”） based on the nucleotide-binding domain leucine-rich repeat and pyrin domain-containing receptor 3 （NLRP3） inflammasome signaling pathway. METHODS Female SD rats were randomly assigned to normal group， model group and Juanxiao decoction low-， medium- and high-dose groups （0.36， 0.72 and 1.44 g/kg， calculated based on crude drug weight）， as well as positive control group （Dexamethasone acetate tablets， 0.2 mg/kg）， with 10 rats in each group. Except for the normal group， asthma models were established in the remaining groups via intraperitoneal injection of ovalbumin combined with aluminum hydroxide， followed by nebulized inhalation of ovalbumin. On day 14 of the experiment， rats in each group received intragastric administration of the corresponding solution or normal saline， once a day， for 7 consecutive days. Following the final administration， the following parameters were measured in each group： lung function indexes （forced vital capacity， forced expiratory volume in 0.3 second， peak expiratory flow）， serum levels of inflammatory markers （interleukin-1β， interleukin- 18）， and the percentages of inflammatory cells （lymphocytes， eosinophils， neutrophils） in bronchoalveolar lavage fluid. Histopathological changes in lung tissue were observed， and the protein and mRNA expressions of nuclear factor-kappa B （NF- κB）， NLRP3 and caspase-1 in lung tissue were detected. RESULTS Compared with the normal group， pathological changes such as alveolar wall thickening and inflammatory cell infiltration were observed in rats in the model group. All pulmonary function indicators were significantly reduced in rats in the model group and the administration groups. The levels of inflammatory markers， the percentages of inflammatory cells， and the protein and mRNA expressions of NF-κB， NLRP3 and caspase-1 were significantly elevated or up-regulated （P＜0.05）. Compared with the model group， pathological changes in rats in each dosage group of Juanxiao decoction were significantly alleviated， and all quantitative indicators showed dose-dependent improvements （P＜0.05）. CONCLUSIONS Juanxiao decoction can reduce airway inflammatory responses in asthmatic rats， alleviate lung function impairment， and improve pathological changes such as inflammatory cell infiltration. Those effects may be related to the inhibition of the NLRP3 inflammasome signaling pathway. |
| 期刊： | 2026年第37卷第02期 |
| 作者： | 曹邦卿；喻强强；刘美年；吴支喜；曾丽珍；童梦瑶；邓云华；薛汉荣 |
| AUTHORS： | CAO Bangqing， YU Qiangqiang，LIU Meinian，WU Zhixi，ZENG Lizhen，TONG Mengyao，DENG Yunhua，XUE Hanrong |
| 关键字： | 蠲哮汤；支气管哮喘；NLRP3炎症小体信号通路 |
| KEYWORDS： | Juanxiao decoction； bronchial asthma； NLRP3 inflammasome signaling pathway |
| 阅读数： | 2 次 |
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