密蒙花花蕾不同提取部位的抗氧化活性研究
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篇名: 密蒙花花蕾不同提取部位的抗氧化活性研究
TITLE:
摘要:

目的:比较密蒙花花蕾不同提取部位的体外抗氧化活性。方法:用60%乙醇提取密蒙花花蕾得乙醇粗提物(ET),用水分

散后,依次用石油醚、乙酸乙酯和正丁醇萃取ET得各部位样品PE、EA、BU和水部位样品(SH);以二丁基羟基甲苯(BHT)为阳性

对照。采用1,1-二苯基-2-苦基苯肼(DPPH)、2,2′-联氨-双(3-乙基苯并噻唑啉-6-磺酸)二氨盐(ABTS)自由基清除法和铁离子还

原/抗氧化能力分析(FRAP)法分别考察各部位样品的抗氧化能力,计算半数抑制浓度(IC50)及抗氧化当量TEAC值。结果:与其

他部位样品比较,ET 与EA 具有较强的抗氧化活性,EA 清除DPPH、ABTS 自由基的IC50分别为13.75 、9.78 μg/ml,ET 为14.93、

11.41 μg/ml;EA对DPPH 自由基的清除能力强于BHT(IC50为18.71 μg/ml)。EA、ET、BHT的TEAC值分别为1 657.67、1 586.25、

1 581.68 μmol/g。结论:密蒙花花蕾的乙酸乙酯提取物具有较好的抗氧化能力。

ABSTRACT:

OBJECTIVE:To compare antioxidant activity of different extraction parts from flower buds of Buddlejae flos in vitro.

METHODS:Ethanol crude extract(ET)was extracted from flower buds of B. flos with 60% ethanol and diffused by water;

and petroleum ether,ethyl acetate and n-butanol were used to extract ET to obtain PE,EA,BU and water samples(SH). Using dibutyl

hydroxy toluene(BHT)as positive control,the antioxidant capacity of PE,EA,BU and SH were investigated by using 1,

1-diphenyl-2-picrylhydrazyl(DPPH),2,2′-azino-bis(3-ethyl benzothiazoline-6-sulfonic acid)diammonium salt(ABTS)free radical

scavenging method and ironion reduction/oxidation resistance ability (FRAP) method. IC50 and antioxdant equivalent TEAC

were calculated. RESULTS:Compared with other samples,ET and EA had stronger antioxidant activity,and IC50 of EA scavenging

DPPH and ABTS free radical were 13.75,9.78 μg/ml,and those of ET were 14.93,11.41 μg/ml;scavenging ability of EA to

DPPH free radical was stronger than that of BHT(IC50 was 18.71 μg/ml). TEAC of EA,ET and BHT were 1 657.67,1 586.25

and 1 581.68 μmol/g. CONCLUSIONS:The ethyl acetate extract from flower buds of B. flos has good antioxidant activity.

期刊: 2016年第27卷第1期
作者: 杨再波,谌连桃,吴应红,贺银菊
AUTHORS: YANG Zaibo,CHEN Liantao,WU Yinghong,HE Yinju
关键字: 密蒙花花蕾;抗氧化活性;自由基;清除率;提取部位
KEYWORDS: Flower buds of Buddlejae flos;Antioxidant activity;Free radical;Scavenging rate;Extract part
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