目的：研究黄芩素对大鼠心肌缺血/再灌注（I/R）损伤的改善作用。方法：采用Langendorff 法建立心肌I/R 损伤模型。取

40 只大鼠离体心脏随机均分为正常组（不停灌处理）、模型组（停灌20 min）和黄芩素高、中、低浓度组（停灌前分别灌注含40、10、

2.5 μmol/ml 黄芩素的K-H液10 min）。检测心肌梗死率，冠脉流出液中肌酸激酶（CK）、乳酸脱氢酶（LDH）的活性，心肌组织中超

氧化物歧化酶（SOD）活性、丙二醛（MDA）含量、还原型谷胱甘肽/氧化型谷胱甘肽（GSH/GSSG）比值以及心肌细胞凋亡率。结果：

与正常组比较，模型组大鼠离体心脏的心肌梗死率、心肌细胞凋亡率、CK和LDH活性升高，心肌组织中MDA含量增加、SOD活

性、GSH/GSSG 比值降低（P＜0.01）。与模型组比较，黄芩素低、中浓度组大鼠离体心脏的心肌梗死率、CK和LDH活性降低，心

肌组织中SOD活性升高（P＜0.05 或P＜0.01），并且中浓度组心肌细胞凋亡率明显降低、心肌组织中MDA含量减少、GSH/GSSG

比值升高（P＜0.05 或P＜0.01）；高浓度组各指标均无明显变化（P＞0.05）。结论：黄芩素对大鼠心肌I/R 损伤具有一定的改善作

用，其机制可能与黄芩素的抗氧化和抗凋亡作用相关。

OBJECTIVE：To study the improvement effects of baicalein against myocardial ischemia/reperfusion（I/R）injury in

rats. METHODS：I/R injury model was induced by Langendorff method. Isolated heart of 40 rats were randomly divided into normal

group（continuous perfusion），model group（perfusion withdrawal 20 min）and baicalein high，medium and low concentration

groups（K-H solution of baicalein 40，10 and 2.5 μmol/ml 10 min before perfusion withdrawal）. The myocardial infarction rate，

the activity of creatine kinase（CK）and lactate dehydrogenase（LDH）in coronary effluent liquid，SOD activity and MDA content，

GSH/GSSG and apoptosis rate of cardiac muscle cell in myocardial tissue were detected. RESULTS：Compared with normal group，

the myocardial infarction rate，apoptosis rate of cardiac muscle cell，the activities of CK and LDH and the content of MDA in myocardial

tissue were increased in model group，while SOD activity and GSH/GSSG of myocardial tissue decreased（P＜0.01）. Compared

with model group，the isolated myocardial infarction rate and the activities of CK and LDH in baicalein low and medium concentration

groups decreased，while SOD activity increased（P＜0.05 or P＜0.01）；apoptosis rate of cardiac muscle cell and the content

MDA of myocardial tissue decreased significantly in medium concentration group，while GSH/GSSG increased（P＜0.05 or

P＜0.01）；those indicators had no significant change in high concentration group（P＞0.05）. CONCLUSIONS：Baicalein has certain

improvement effect on myocardial I/R injury in rats，and its mechanism may be associated with antioxidant and anti-apoptosis

effect of baicalein.