陵水暗罗根超临界CO2萃取物的GC-MS分析及其体外生物活性
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篇名: 陵水暗罗根超临界CO2萃取物的GC-MS分析及其体外生物活性
TITLE:
摘要:

:提取、鉴定陵水暗罗根提取物中挥发性成分,并评价其体外生物活性。方法:采用超临界CO2萃取法提取陵水暗罗

根中的挥发性化学成分,气相色谱-质谱联用(GC-MS)法分析其化学成分组成;以0(空白对照)、5、10、20、30、40、50 μg(生药)/ml

提取物培养人肝癌Huh-7 细胞24 h 后,采用MTT法测定其对细胞的抑制作用,计算相对细胞活力和半数抑制浓度(IC50);分别采

用纸片琼脂扩散法和肉汤稀释法测定其对金黄色葡萄球菌、耐甲氧西林金黄色葡萄球菌和粪肠球菌等菌株的体外抗菌活性。结

果:从陵水暗罗根超临界CO2萃取物中分离鉴定了40 个化合物,主要成分为(Z,Z,Z)-9,12,15-octa-decatrien-1-ol、甘油、肉桂醛、棕

榈酸和丁香酚等;与空白对照组比较,陵水暗罗根提取物在5 μg(生药)/ml 以上质量浓度时对细胞生长有显著抑制作用(P<

0.05),且随着质量浓度的增加抑制作用增强,IC50值为5.2 μg(生药)/ml;两种抑菌试验均未检测出其具有抑菌活性。结论:超临界

CO2萃取法和GC-MS法能有效提取和鉴定陵水暗罗根超临界CO2萃取物的挥发性成分;提取物表现出抑制细胞活力的作用,但无

抗菌活性。

ABSTRACT:

OBJECTIVE:To extract and identify the volatile components of the roots of Polyalthia nemoralis,and to evaluate

its biological activity in vitro. METHODS:Supercritical CO2 extraction(SFE-CO2)was performed to extract volatile compounds

from the roots of P. nemoralis,and the volatile components were separated and determined by GC-MS. Human hepatic cancer

Huh-7 cells were cultured with 0(blank control),5,10,20,30,40 and 50 μg(medicinal material)/ml extract for 24 h,and then

MTT assay was used to detect the inhibitory effect of the extracts on cells. Relative cell viability and IC50 were calculated. The anti-

bacterial activities of extract to Staphylococcus aureus,Methicillin-resistant Staphylococcus aureus,Enterococcus faecalis and other

strain were determined by Kirby-bauer method and broth dilution method. RESULTS:Forty compounds were identified from the

SFE-CO2 extracts from the roots of P. nemoralis. The main constituents were(Z,Z,Z)-9,12,15-octa-decatrien-1-ol,glycerin,cinnamaldehyde,

n-hexadecanoic acid and eugenol. Compared with blank control group,SFE-CO2 extracts from the roots of P. nemoralis

5 μg(medicinal material)/ml and above showed significant inhibitory effect on cell growth(P<0.05),and the inhibitory effect was

strengthened as the concentration of extracts increased,IC50 values was 5.2 μg(medicinal material)/ml. However,the supercritical

extract didn’t showed antibacterial activity against three microorganisms in 2 kinds of antibacterial tests. CONCLUSIONS:

SFE-CO2 and GC-MS method can effectively extract and identify volatile components of the roots of P. nemoralis,and supercritical

extracts can inhibit the viability of cells but have no antibacterial activity.

期刊: 2016年第27卷第1期
作者: 黄冬苑,刘嘉炜,李武国,林伟杰
AUTHORS: HUANG Dongyuan,LIU Jiawei,LI Wuguo,LIN Weijie
关键字: 陵水暗罗;挥发性成分;超临界CO2萃取;气相色谱-质谱联用;抑制作用;抗菌活性
KEYWORDS: Polyalthia nemoralis;Volatile components;Supercritical CO2 extraction;GC-MS;Inhibitory effect;Antibacterial
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