HPLC法同时测定桂朴微丸中厚朴酚、和厚朴酚的含量
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篇名: | HPLC法同时测定桂朴微丸中厚朴酚、和厚朴酚的含量 |
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摘要: | 目的:建立同时测定桂朴微丸中厚朴酚、和厚朴酚含量的方法。方法:采用高效液相色谱法。色谱柱为Alltima C18,流动相为甲醇-水 (70 ∶ 30,V/V),流速为1.0 ml/min,检测波长为294 nm,柱温为35 ℃,进样量为10 μl。结果:厚朴酚、和厚朴酚检测进样量线性范围分别为0.206 4~2.064 μg(r=0.999 8)、0.088 0~0.880 μg(r=0.999 9);定量限分别为0.41、0.26 μg/ml,检测限分别为0.12、0.075 μg/ml;精密度、稳定性、重复性试验的RSD<2.0%;加样回收率分别为99.86%~102.11%(RSD=1.02%,n=6)、96.53%~101.74%(RSD=1.83%,n=6)。结论:该方法准确、灵敏、重复性好,可用于同时测定桂朴微丸中厚朴酚、和厚朴酚的含量。 |
ABSTRACT: | OBJECTIVE: To establish a method for simultaneous determination of magnolol and honokiol in Guipo pellet. METHODS: HPLC was performed on the column of Alltima C18 with mobile phase of methanol-water (70 ∶ 30,V/V) at a flow rate at 1.0 ml/min, detection wavelength was 294 nm, column temperature was 35 ℃ and injection volume was 10 μl. RESULTS: The linear range was 0.206 4-2.064 μg for magnolol (r=0.999 8) and 0.088 0-0.880 μg for honokiol (r=0.999 9); limits of quantitation were 0.41, 0.26 μg/ml, detection limits were 0.12, 0.075 μg/ml, respectively; RSDs of precision, stability and reproducibility tests were lower than 2.0%; recoveries were 99.86%-102.11% (RSD=1.02%,n=6) and 96.53%-101.74% (RSD=1.83%,n=6). CONCLUSIONS: The method is accurate, sensitive, reproducible, and suitable for the simultaneous determination of magnolol and honokiol in Guipo pellet. |
期刊: | 2016年第27卷第33期 |
作者: | 赵呈雷,黄一平,田耀洲,曹鹏 |
AUTHORS: | ZHAO Chenglei,HUANG Yiping,TIAN Yaozhou,CAO Peng |
关键字: | 桂朴微丸;厚朴酚;和厚朴酚;高效液相色谱法;含量测定 |
KEYWORDS: | Guipo pellet; Magnolol; Honokiol; HPLC; Content determination |
阅读数: | 333 次 |
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