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辛芍组方对氧糖剥夺损伤的神经PC12细胞凋亡的影响及其机制研究
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| 篇名： | 辛芍组方对氧糖剥夺损伤的神经PC12细胞凋亡的影响及其机制研究 |
| TITLE： | |
| 摘要： | 目的：考察辛芍组方对氧糖剥夺（OGD） 损伤的神经PC12细胞凋亡的影响，并探讨其作用机制。方法：采用连二亚硫酸钠培养PC12细胞建立OGD损伤模型，将OGD损伤的细胞分为模型组（空白培养基）、尼莫地平组（阳性药物，0.42 mg/L）和辛芍组方低、中、高浓度组[质量浓度分别为0.313、0.625、1.25 mg（生药）/L]，并取正常细胞作为正常对照组（空白培养基）。各组细胞经药物作用4 h，再复糖复氧4 h后采用MTT法检测细胞存活率，比色法测定乳酸脱氢酶（LDH）的释放量，流式细胞术检测细胞凋亡率和线粒体膜电位（MMP），并采用Western blot法检测细胞凋亡相关基因Caspase-3、Bax、Bcl-2蛋白的表达。结果：与正常对照组比较，模型组细胞的存活率、MMP、Bcl-2蛋白表达水平以及Bcl-2/Bax比值显著降低，而LDH释放量、细胞凋亡率以及Caspase-3、Bax蛋白表达水平显著升高 （P＜0.05或P＜0.01）。与模型组比较，辛芍组方可增加细胞的存活率和MMP，降低LDH的释放量和凋亡率，显著抑制Caspase-3和Bax蛋白表达水平，升高Bcl-2蛋白表达水平和Bcl-2/Bax比值；除辛芍组方低浓度组细胞的MMP、Bcl-2和Bax外，其余各给药组细胞上述指标差异均有统计学意义（P＜0.05或P＜0.01）。结论：辛芍组方对OGD损伤诱导的PC12细胞凋亡具有抑制作用，其机制可能与减少LDH释放、稳定细胞MMP、调节凋亡相关基因的表达有关。 |
| ABSTRACT： | OBJECTIVE： To investigate the effects of Xinshao formula on the apoptosis of oxygen-glucose deprivation （OGD）-induced PC12 cells， and to investigate its mechanism. METHODS： PC12 cells were cultured by Na2S2O4 to establish OGD model. OGD-induced cells were divided into model group （blank medium）， nimodipine group （positive drug， 0.42 mg/L） and Xinshao formula low-concentration， medium-concentration and high-concentration groups [0.313， 0.625， 1.25 mg （crude drug）/L]； normal cells were included in normal control group （blank medium）. MTT assay was used to determine the survival rate of PC12 cells after treated with drugs for 4 h and then oxygen-glucose restoration for 4 h. The release of LDH was detected by colorimetric method. The apoptosis rate and mitochondrial membrane potential （MMP） were detected by flow cytometry. The protein expression of Caspase-3， Bax and Bcl-2 were examined by Western blot. RESULTS： Compared with normal control group， the survival rate， MMP， the protein expression of Bcl-2 and the ratio of Bcl-2/Bax were decreased significantly in model group； while the release of LDH， apoptosis rate， protein expression of Caspase-3 and Bax were increased significantly （P＜0.05 or P＜0.01）. Compared with model group， the survival rate， MMP， the protein expression of Bcl-2 and the ratio of Bcl-2/Bax were increased in Xinshao formula groups； while the release of LDH， apoptosis rate， protein expression of Caspase-3 and Bax were decreased. The above indexes had significant differences except for MMP， Bcl-2 and Bax in Xinshao formula low-concentration group （P＜0.05 or P＜0.01）. CONCLUSIONS： Xinshao formula can inhibit the apoptosis of OGD-induced PC12 cells， which may be related to reducing the release of LDH， keeping MMP stable and regulating the expression of apoptotic-related gene. |
| 期刊： | 2016年第27卷第28期 |
| 作者： | 董永喜，董莉，付思红，刘宗炎，王爱民，李勇军，兰燕宇 |
| AUTHORS： | DONG Yongxi，DONG Li，FU Sihong，LIU Zongyan，WANG Aimin，LI Yongjun，LAN Yanyu |
| 关键字： | 辛芍组方；氧糖剥夺；神经PC12细胞；凋亡；凋亡相关基因；线粒体膜电位 |
| KEYWORDS： | Xinshao formula； Oxygen-glucose deprivation； Nerve PC12 cells； Apoptosis； Apoptotic-related gene； Mitochondrial membrane potential |
| 阅读数： | 351 次 |
| 本月下载数： | 3 次 |
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