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Box-Behnken响应面法优化通关藤总皂苷的超声提取工艺及其纯化工艺研究
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| 篇名： | Box-Behnken响应面法优化通关藤总皂苷的超声提取工艺及其纯化工艺研究 |
| TITLE： | |
| 摘要： | 目的：优化通关藤中总皂苷的超声提取工艺及纯化工艺，制备纯化的通关藤总皂苷。方法：以通关藤总皂苷提取率为指标，以乙醇体积分数、液料比和提取温度等为因素，采用单因素试验和Box-Behnken响应面法优化通关藤总皂苷的超声提取工艺；以洗脱液中总皂苷含量为指标，考察乙醇洗脱体积分数对总皂苷经D101型大孔吸附树脂吸附纯化工艺的影响。结果：最优超声提取工艺条件为乙醇体积分数85％、液料比18.5 ∶ 1（ml/g）、提取温度80 ℃；验证试验中通关藤总皂苷平均提取率为4.80％（RSD＝1.06％，n＝3），与预测值4.89％接近，偏差为1.84％。最优纯化工艺条件为以45％乙醇为洗脱溶剂除杂，再用90％乙醇进行纯化；验证试验表明通关藤总皂苷含量实测平均值为62.45％（RSD＝0.88％，n＝3）。结论：优化后的工艺可较好地提取、纯化通关藤中的总皂苷，且工艺稳定、可靠。 |
| ABSTRACT： | OBJECTIVE： To optimize the ultrasonic extraction and purification technology of total saponins from Marsdenia tenacissima， and to prepare purified total saponins from M. tenacissima. METHODS： Using the extraction rate of total saponins from M. tenacissima as index， ethanol volume fraction， solvent-solid ratio and extraction temperature as factors， ultrasonic extraction technology of total saponins from M. tenacissima was optimized by single factor test and Box-Behnken response surface design. Using the content of total saponins in elution， the effects of ethanol elution volume fraction on purification technology of total saponins by D101 type macroporous adsorption resin was investigated. RESULTS： The optimal ultrasonic extraction technology was as follows as ethanol volume fraction of 85％， solvent-solid ratio of 18.5 ∶ 1 （ml/g）， extraction temperature of 80 ℃. In verification test， the average extraction rate of total saponins from M. tenacissima was 4.80％ （RSD＝1.06％， n＝3）； it was close to predicted value 4.89％， and the deviation was 1.84％. The optimal purification technology was as follows as 45％ ethanol as eluant， 90％ ethanol for purification. Verification test showed that the average content of total saponins from M. tenacissima was 62.45％ （RSD＝0.88％， n＝3）. CONCLUSIONS： The optimized technology can be used for the extraction and purification of total saponins from M. tenacissima. The technology is stable and reliable. |
| 期刊： | 2016年第27卷第25期 |
| 作者： | 徐东伟，王绍辉，刘同祥 |
| AUTHORS： | XU Dongwei，WANG Shaohui，LIU Tongxiang |
| 关键字： | 通关藤；总皂苷；提取工艺；Box-behnken响应面法；大孔吸附树脂；纯化 |
| KEYWORDS： | Marsdenia tenacissima；Total saponins； Extraction technology； Box-Behnken response surface method； Macroporous adsorption resin； Purification |
| 阅读数： | 248 次 |
| 本月下载数： | 3 次 |
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