黄芪颗粒对病毒性心肌炎模型大鼠心肌细胞中Cav-3、Smad3表达的影响-中国药房

黄芪颗粒对病毒性心肌炎模型大鼠心肌细胞中Cav-3、Smad3表达的影响

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篇名：	黄芪颗粒对病毒性心肌炎模型大鼠心肌细胞中Cav-3、Smad3表达的影响
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摘要：	目的：研究黄芪颗粒对病毒性心肌炎模型大鼠心肌细胞中小凹蛋白3（Cav-3）和Smad家族蛋白3（Smad3）表达的影响。方法：将90只大鼠随机分为正常组、模型组、参麦注射液组[阳性药物，0.2 g/（kg·d）]和黄芪颗粒低、中、高剂量组[0.42、0.84、1.68 g/（kg·d）]，每组15只。除正常组外，其余各组大鼠均ip CVB3病毒复制病毒性心肌炎模型。造模同时各给药组大鼠ig相应药物，正常组和模型组大鼠ig生理盐水，连续15 d。各组分别于给药3、9、15 d后取5只大鼠进行实验，观察大鼠心肌组织病理改变，并进行评分；检测心肌细胞中Cav-3、Smad3 mRNA及其蛋白的表达。结果：给药15 d 后，与正常组比较，模型组大鼠心肌纤维大量增生，发生明显病变，病理评分和心肌细胞中Cav-3、Smad3 mRNA及其蛋白水平明显升高（P＜0.05）；与模型组比较，各给药组大鼠心肌组织病变得到改善，病理评分和心肌细胞中Cav-3、Smad3 mRNA及其蛋白水平明显降低（P＜0.05）。结论：黄芪多糖可明显下调病毒性心肌炎模型大鼠心肌细胞中Cav-3、Smad3基因的表达，推测是其防治病毒性心肌炎的作用机制之一。
ABSTRACT：	OBJECTIVE： To study the effects of Astragalus granules on the expression of Caveolin-3 （Cav-3） and Smad family member 3 （Smad3） in the myocardial cells of rats with viral myocarditis. METHODS： 90 rats were randomly divided into a normal group， a model group， a Shenmai injection group [positive drug， 0.2 g/（kg·d）] and the groups of low， medium and high-dose Astragalus granules [0.42， 0.84， 1.68 g/（kg·d）]， with 15 rats in each group. The rats in all groups except for the normal group were given CVB3 ip for the establishment of viral myocarditis model. Meanwhile， the rats in the drug administration groups were given corresponding drugs ig， while those in the normal group and the model group were given normal saline ig， for 15 consecutive days. 5 rats were selected from each group respectively on the 3rd， 9th and 15th days of drug use to take an experiment. For the rats， the pathological change of the cardiac muscle tissue was observed and scored， and the mRNA and protein expression of Cav-3 and Smad3 in the myocardial cells were detected. RESULTS： After 15 days of drug use， compared to the normal group， the rats of the model group had hyperplasia of a large number of cardiac muscle fibers， obvious lesions at cardiac muscle fibers， and significantly higher pathological score and levels of the mRNA and protein expression of Cav-3 and Smad3 in the myocardial cells （P＜0.05）. Compared to the model group， the rats in the drug administration groups had cardiac muscle tissue lesions improved and had obviously lower pathological score and levels of the mRNA and protein expression of Cav-3 and Smad3 in the myocardial cells （P＜0.05）. CONCLUSIONS： Astragalus granules can markedly downregulate the gene expression of Cav-3 and Smad3 in the myocardial cells of rats with viral myocarditis， which is inferred as a prevention and treatment mechanism of viral myocarditis.
期刊：	2016年第27卷第25期
作者：	李丽燕，俞金正，沈群
AUTHORS：	LI Liyan，YU Jinzheng，SHEN Qun
关键字：	黄芪颗粒；病毒性心肌炎；小凹蛋白3；Smad家族蛋白3；大鼠
KEYWORDS：	Astragalus granules； Viral myocarditis； Caveolin-3； Smad family member 3； Rats
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