调经助孕胶囊对着床障碍模型小鼠子宫内膜容受性的影响及其机制研究-中国药房

调经助孕胶囊对着床障碍模型小鼠子宫内膜容受性的影响及其机制研究

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篇名：	调经助孕胶囊对着床障碍模型小鼠子宫内膜容受性的影响及其机制研究
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摘要：	目的：研究调经助孕胶囊对着床障碍模型小鼠子宫内膜容受性的影响及其机制。方法：将妊娠小鼠随机分为正常组、模型组和调经助孕胶囊低、高剂量组[12、24 g/（kg·d）]，每组10只。从妊娠第1天开始，各给药组小鼠ig相应药物，正常组和模型组小鼠ig生理盐水，连续3 d；妊娠第4天，除正常组外，其余各组小鼠均ih米非司酮溶液复制着床障碍模型；妊娠第5天处死小鼠。观察子宫内膜组织形态变化，并对子宫内膜成熟度进行评分；检测子宫内膜白血病抑制因子（LIF）、同源框基因HOXA10、基质金属蛋白酶9（MMP-9）、基质金属蛋白酶抑制因子1（TIMP-1）蛋白表达水平。结果：与正常组比较，模型组和各给药组小鼠子宫内膜发育均不完全，成熟度评分和子宫内膜LIF、HOXA10、MMP-9、TIMP-1蛋白表达水平均明显降低（P＜0.05）。与模型组比较，给药组小鼠子宫内膜发育得到改善或趋向正常，各指标水平均明显升高（P＜0.05）；且调经助孕胶囊高剂量组指标改善较低剂量组更明显（P＜0.05）。结论：调经助孕胶囊可改善着床障碍小鼠的子宫内膜容受性；其机制可能与上调HOXA10、LIF基因的表达，维持MMP-9、TIMP-1平衡有关。
ABSTRACT：	OBJECTIVE： To investigate the effects of Tiaojingzhuyun capsules on endometrial receptivity in mice with implantation dysfunction and corresponding mechanism. METHODS： Pregnant mice were randomized into normal group， model group and the groups of low and high-dose [12， 24 g/（kg·d）] Tiaojingzhuyun capsules， with 10 mice in each group. The mice in the drug administration groups were given corresponding drugs intragastrically， while those in the normal group and the model group were given normal saline intragastrically， for 3 consecutive days from the 1st day of pregnancy. On the 4th day of pregnancy， the mice in all groups except for the normal group were given mifepristone solution subactaneously to establish model of implantation dysfunction. On the 5th day of pregnancy， the mice were sacrificed， and then the change in endometrial morphology was observed and endometrial maturity was scored； the protein expression levels of leukaemia inhibitory factor （LIF）， homoeobox gene HOXA10， matrix metalloproteinase 9 （MMP-9） and matrix metalloproteinase inhibitory factor 1 （TIMP-1） expression in endometrial tissues were determined. RESULTS： Compared to the normal group， the mice in the model group and the drug administration groups had endometrial hypoplasia and obviously lower endometrial maturity score and protein expression levels of LIF， HOXA10， MMP-9 and TIMP-1 in endometrial tissues （P＜0.05）. Compared to the model group， the mice in the drug administration groups demonstrated endometrial development which improved or tended to be normal and had significantly higher indexes mentioned above （P＜0.05）； besides， the effect shown in the group of high-dose Tiaojingzhuyun capsules was stronger than that in the group of low-dose Tiaojingzhuyun capsules （P＜0.05）. CONCLUSIONS： Tiaojingzhuyun capsules can improve the endometrial receptivity in mice with implantation dysfunction by a mechanism which may be related to upregulating the gene expression of HOXA10 and LIF， and maintaining the balance of MMP-9 and TIMP-1.
期刊：	2016年第27卷第25期
作者：	王瑞杰
AUTHORS：	WANG Ruijie
关键字：	调经助孕胶囊；着床障碍；子宫内膜容受性；成熟度评分；机制；小鼠
KEYWORDS：	Tiaojingzhuyun capsules； Implantation dysfunction； Endometrial receptivity； Maturity score； Mechanism； Mice
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